Polyethyleneimine-modified calcium carbonate nanoparticles for p53 gene delivery.
Polyethyleneimine-modified calcium carbonate nanoparticles for p53 gene delivery.
复制标题
用于 p53 基因传递的聚乙烯亚胺修饰碳酸钙纳米颗粒
DOI：
10.1093/rb/rbv029
复制
发表时间：
2016-03
影响因子：
6.7
通讯作者：
Kong X
中科院分区：
文献类型：
--
作者：
Chen C;Han H;Yang W;Ren X;Kong X
本研究以精氨酸为调节剂，采用共沉淀法成功制备了球形碳酸钙纳米粒子。所制备的CaCO 3的平均粒径约为900 nm。采用扫描电镜、傅里叶变换红外光谱、X射线衍射和粒度分布等方法对纳米碳酸钙的性质进行了表征。研究了聚乙烯亚胺（PEI）修饰的PEI-CaCO 3纳米粒携带绿色荧光蛋白（GFP）标记的p53基因（pEGFP-C1-p53）进入肿瘤细胞表达P53蛋白的能力。同时，通过细胞毒性、转染效率、细胞生长抑制和P53蛋白诱导细胞凋亡的能力来评价PEI-CaCO 3纳米粒的性能。结果表明，制备的PEI-CaCO 3纳米粒子在一定浓度范围内具有良好的生物相容性和较低的细胞毒性。PEI-CaCO 3有效地将pEGFP-C1基因转染到上皮样癌细胞中。并且随着GFP-P53融合蛋白的表达，负载pEGFP-C1-p53基因的PEI-CaCO 3颗粒明显抑制了癌细胞的增殖。这些发现表明，我们的PEI修饰的碳酸钙纳米粒子是潜在的成功地用作基因治疗的载体。
In this study, calcium carbonate (CaCO3) nanoparticles with spherical structure were regulated by arginine and successfully synthesized via a facile co-precipitation method. The average particle size of as-prepared CaCO3 was about 900 nm. The properties of nanostructured CaCO3 particles were characterized by scanning electron microscope, Fourier transform infrared spectroscopy, X-ray diffraction and size distribution. After modified with polyethyleneimine (PEI), the ability of PEI-CaCO3 nanoparticles to carry GFP-marked p53 gene (pEGFP-C1-p53) into cancer cells to express P53 protein were studied. Meanwhile, the cytotoxicity, transfection efficiency, cells growth inhibition and the ability to induce apoptosis by expressed P53 protein were conducted to evaluate the performances of PEI-CaCO3 nanoparticles. The results show that prepared PEI-CaCO3 nanoparticles had good biocompatibility and low cytotoxicity in a certain concentration range. PEI-CaCO3 effectively transfected pEGFP-C1 gene into epithelial-like cancer cells. And with the expression of GFP-P53 fusion protein, pEGFP-C1-p53-gene-loaded PEI-CaCO3 particles significantly reduced the proliferation of cancer cells. These findings indicate that our PEI-modified CaCO3 nanoparticles are potential to be successfully used as carriers for gene therapy.