Epidemiological Study on Newly Emerging Lactococcus garvieae Serotype II Isolated from Marine Fish Species in Japan
Epidemiological Study on Newly Emerging Lactococcus garvieae Serotype II Isolated from Marine Fish Species in Japan
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DOI：
10.3147/jsfp.54.51
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发表时间：
2019-09-01
期刊：
影响因子：
0.6
通讯作者：
Yoshida, Terutoyo
中科院分区：
文献类型：
--
作者：
Shi, Yin-Ze;Nishiki, Issei;Yoshida, Terutoyo
Garvieae血清型II是一种新出现的乳球菌感染致病病原体，自2012年以来已蔓延到养鱼场，其对水产养殖业的损害有所增加。从黄尾、五斑、琥珀、黄鳍等4种鱼类中共分离到161株细菌。dumerili，皇带鱼S. 2012年至2017年间，在8个县发现了拉兰迪（lalandi）和条纹鳕（Pseudocaranx dentex）。这是首次对L.通过抗血清凝集试验、偏置正弦场凝胶电泳（BSFGE）分析和药敏试验，对2型鸡瘟病菌进行了初步鉴定。这些菌株几乎没有一个与抗-la血清（抗-KG-血清）凝集，除了两个菌株与抗-la和II血清凝集。BSFGE分析表明，遗传上同源的L.自2012年首次爆发以来，Garvieae血清型II已蔓延到养鱼场，并且在2015年已经发现了对林可霉素具有耐药性的菌株。
Lactococcus garvieae serotype II, a newly emerging causal pathogen of lactococcal infection, has spread to fish farms since 2012, and its damage to aquaculture has increased. A total of 161 strains were isolated from four fish species, yellowtail Seriola quinqueradiata, amberjack S. dumerili, kingfish S. lalandi, and striped jack Pseudocaranx dentex, in eight prefectures between 2012 and 2017. This is the first epidemiological study on L. garvieae serotype II, by using agglutination titers against antisera for diagnosis, biased sinusoidal field gel electrophoresis (BSFGE) analysis, and antimicrobial susceptibility test. Almost none of these strains were agglutinated with anti-la serum (anti-KG - serum), except for two strains agglutinated with both anti-la and II sera. BSFGE analysis revealed that genetically homogeneous L. garvieae serotype II has spread to fish farms since the first outbreak in 2012, and the strains resistant to lincomycin were already found in 2015.