Development of a Biosensor Based on Angiotensin-Converting Enzyme II for Severe Acute Respiratory Syndrome Coronavirus 2 Detection in Human Saliva.
Development of a Biosensor Based on Angiotensin-Converting Enzyme II for Severe Acute Respiratory Syndrome Coronavirus 2 Detection in Human Saliva.
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DOI：
10.3389/fsens.2022.917380
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发表时间：
2022-01-01
期刊：
影响因子：
--
通讯作者：
Vanegas, Diana
中科院分区：
文献类型：
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作者：
Moreira, Geisianny;Casso-Hartmann, Lisseth;Vanegas, Diana
严重急性呼吸综合征冠状病毒2（SARS-CoV-2）是导致COVID-19的新型冠状病毒。人类感染需要血管紧张素转换酶II（hACE 2）作为SARS-CoV-2的进入点。PCR检测通常是明确的，但昂贵，虽然它是高度敏感和准确的。基于生物传感器的监测可能是一种低成本，准确和非侵入性的方法，以提高检测能力。我们开发了一种用于检测唾液中完整SARS-CoV-2的电容式hACE 2生物传感器。用铂纳米颗粒修饰激光诱导石墨烯（LIG）电极。使用循环伏安法进行LIG电极的质量控制。截短的hACE 2被用作生物识别元件，并通过链霉亲和素-生物素偶联附着到电极表面。生物层干涉法用于hACE 2与UV衰减病毒体的定性相互作用筛选。电化学阻抗谱（EIS）用于信号转导。截短的hACE 2与野生型SARS-CoV-2及其变体的结合亲和力大于人冠状病毒（普通感冒病毒）。检测限（LoD）估计为2，960拷贝/毫升。检测过程通常需要不到30分钟。这些功能的强度使hACE 2生物传感器成为一种潜在的低成本方法，用于在非临床环境中筛查SARS-CoV-2，快速检测需求很高（例如，学校和机场）。
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the novel coronavirus responsible for COVID-19. Infection in humans requires angiotensin-converting enzyme II (hACE2) as the point of entry for SARS-CoV-2. PCR testing is generally definitive but expensive, although it is highly sensitive and accurate. Biosensor-based monitoring could be a low-cost, accurate, and non-invasive approach to improve testing capacity. We develop a capacitive hACE2 biosensor for intact SARS-CoV-2 detection in saliva. Laser-induced graphene (LIG) electrodes were modified with platinum nanoparticles. The quality control of LIG electrodes was performed using cyclic voltammetry. Truncated hACE2 was used as a biorecognition element and attached to the electrode surface by streptavidin-biotin coupling. Biolayer interferometry was used for qualitative interaction screening of hACE2 with UV-attenuated virions. Electrochemical impedance spectroscopy (EIS) was used for signal transduction. Truncated hACE2 binds wild-type SARS-CoV-2 and its variants with greater avidity than human coronavirus (common cold virus). The limit of detection (LoD) is estimated to be 2,960 copies/ml. The detection process usually takes less than 30 min. The strength of these features makes the hACE2 biosensor a potentially low-cost approach for screening SARS-CoV-2 in non-clinical settings with high demand for rapid testing (for example, schools and airports).