A method for quantitative characterization of growth in the 3-D structure of rat pulmonary arteries
A method for quantitative characterization of growth in the 3-D structure of rat pulmonary arteries
复制标题
DOI：
10.1016/j.mvr.2011.12.003
复制
发表时间：
2012-03-01
影响因子：
3.1
通讯作者：
Feinstein, Jeffrey A.
中科院分区：
文献类型：
--
作者：
Razavi, Hedi;Dusch, Marie N.;Feinstein, Jeffrey A.
了解引起肺血管疾病（PVD）的机制往往需要彻底了解肺循环的潜在结构变化。动物模型已被广泛用于研究不同形式的PVD，但传统的实验技术在一次研究整个肺血管的能力上受到限制。在这项研究中，我们引入了新的动脉铸造技术，高分辨率成像和树分析来研究啮齿动物模型的肺循环。雄性Sprague-Dawley大鼠分别于20、36、52、100和160日龄进行实验。采用显微硅树脂聚合物动脉铸型、12.5 μ m分辨率的高分辨率微计算机断层扫描（micro-CT）和图像数据分析（包括分割和骨架化），可以定性和定量地描述肺血管随年龄增长的变化。影响肺动脉铸造质量的参数包括聚合物流速、注射总量、聚合物粘度和聚合时间。通过优化这些参数，我们成功地建立了不同年龄大鼠的肺循环动脉模型，并证明了定量表征血管数量随出生后生长变化的可行性。这些初步数据表明，在52至100日龄期间，双肺中管腔直径为50200 pm的血管数量迅速增加。有了这项新技术，现在可以在全肺动物模型中研究肺血管系统，以更好地了解疾病对血管结构的整体影响。(C) 2011爱思唯尔公司版权所有。
Understanding mechanisms causing pulmonary vascular disease (PVD) frequently requires a thorough understanding of the underlying structural changes in the pulmonary circulation. Animal models have been used extensively to study different forms of PVD but conventional experimental techniques are limited in their ability to allow the study of the whole pulmonary vasculature at once. In this study, we introduce novel techniques of arterial casting, high-resolution imaging and tree analysis to study the pulmonary circulation in rodent models. Male Sprague-Dawley rats were used at 20, 36, 52, 100 and 160 days of age. A technique involving arterial casting with Microfil silicone polymer, high-resolution micro-Computed Tomography (micro-CT) at 12.5 mu m resolution and image data analysis involving segmentation and skeletonization was developed to both qualitatively and quantitatively describe the changes in the pulmonary vasculature with increasing age. Parameters identified to affect the quality of pulmonary arterial casting included polymer flow rate, total injected volume, polymer viscosity and polymerization time. By optimizing these parameters, we successfully created arterial casts of the pulmonary circulation in rats of different ages and demonstrated the feasibility of quantitatively characterizing the changes in the number of vessels with postnatal growth. These preliminary data suggest that the number of vessels with lumen diameters of 50200 pm increases rapidly in both lungs between 52 and 100 days of age. With this new technique, the pulmonary vasculature can now be studied in a whole lung animal model to better understand the global effects of disease on vascular structure. (C) 2011 Elsevier Inc. All rights reserved.