Femtomolar detection of autoantibodies by magnetic relaxation nanosensors
Femtomolar detection of autoantibodies by magnetic relaxation nanosensors
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DOI：
10.1016/j.ab.2009.05.034
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发表时间：
2009-09-01
影响因子：
2.9
通讯作者：
Prosperi, Davide
中科院分区：
文献类型：
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作者：
Colombo, Miriam;Ronchi, Silvia;Prosperi, Davide
应用于流体样品中生物标志物的快速和灵敏测量的纳米系统的开发是诊断生物医学的当前主要目标。在这篇文章中，我们报告了准确和可靠的检测抗-HSA（人血清白蛋白）抗体的蛋白质功能化的磁性纳米球探针由于其微聚集状态的可逆性改变引起的蛋白质抗体特异性相互作用，作为周围的水分子的T-2弛豫时间的变化。一旦调整了最佳参数，该方法被证明是非常敏感的，提供浓度和时间依赖性的响应。此外，我们证明，所开发的免疫测定能够从T-2线性相关性定量测定生物标志物浓度，从而提供快速，但准确的测定，灵敏度在飞摩尔范围内。这些磁性纳米颗粒的高敏感性和稳定性，以及它们的合成制备，使这些纳米传感器成为一种有前途的新工具，用于多功能和有效的医学诊断。(c)2009 Elsevier Inc. All rights reserved.
The development of nanosystems applied to rapid and sensitive measurement of biomarkers in fluid samples is a current major goal in diagnostic biomedicine. In this article, we report the accurate and reliable detection of anti-HSA (human serum albumin) antibodies by protein-functionalized magnetic nanospherical probes due to the reversible alteration of their microaggregation state induced by protein antibody-specific interaction, sensed as changes in the T-2 relaxation time of surrounding water molecules. Once the optimal parameters were adjusted, the method proved to be very sensitive, providing concentration- and time-dependent responses. Furthermore, we demonstrate that the developed immunoassay is able to quantitatively determine the biomarker concentration from T-2 linear correlation, thereby supplying a rapid, yet accurate, assay with sensitivity in the femtomolar range. The high susceptibility and stability of these magnetic nanoparticles, as well as their accessible synthetic preparation, make these nanosensors a promising new tool for versatile and effective medical diagnostics. (c) 2009 Elsevier Inc. All rights reserved.