MiR-152 Regulates Apoptosis and Triglyceride Production in MECs via Targeting ACAA2 and HSD17B12 Genes.
MiR-152 Regulates Apoptosis and Triglyceride Production in MECs via Targeting ACAA2 and HSD17B12 Genes.
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MiR-152 调节 MEC 中的细胞凋亡和甘油三酯的产生
DOI：
10.1038/s41598-017-18804-x
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发表时间：
2018-01-11
影响因子：
4.6
通讯作者：
Zhao Z
中科院分区：
文献类型：
--
作者：
Yang Y;Fang X;Yang R;Yu H;Jiang P;Sun B;Zhao Z
乳房上皮细胞(MECs)在哺乳期影响产奶量，对维持组织内环境平衡至关重要。我们以前的研究表明，miR-152在高产奶牛的微血管内皮细胞中的表达显著增加。在本研究中，生物信息学分析确定ACAA2和HSD17B12为miR-152的潜在靶点，并通过双荧光素酶报告基因分析进一步验证。此外，定量聚合酶链式反应和Western BOT分析显示miR-152的表达与ACAA2、HSD17B12基因的表达水平呈负相关。此外，miR-152基因可显著上调MEC甘油三酯的生成，促进细胞增殖，抑制细胞凋亡。此外，过表达ACAA2和HSD17B12可抑制甘油三酯的生成、细胞增殖和诱导细胞凋亡，而sh234-ACAA2-181/sh234-HSD17B12-474可逆转这一趋势。这些结果表明miR-152可能通过靶基因ACAA2和HSD17B12的表达而显著影响甘油三酯的产生和抑制细胞凋亡。
Mammary epithelial cells (MECs) affect milk production capacity during lactation and are critical for the maintenance of tissue homeostasis. Our previous studies have revealed that the expression of miR-152 was increased significantly in MECs of cows with high milk production. In the present study, bioinformatics analysis identified ACAA2 and HSD17B12 as the potential targets of miR-152, which were further validated by dual-luciferase repoter assay. In addition, the expressions of miR-152 was shown to be negatively correlated with levels of mRNA and protein of ACAA2, HSD17B12 genes by qPCR and western bot analysis. Furthermore, transfection with miR-152 significantly up-regulated triglyceride production, promoted proliferation and inhibited apoptosis in MECs. Furthermore, overexpression of ACAA2 and HSD17B12 could inhibit triglyceride production, cells proliferation and induce apoptosis; but sh234-ACAA2-181/sh234-HSD17B12-474 could reverse the trend. These findings suggested that miR-152 could significantly influence triglyceride production and suppress apoptosis, possibly via the expression of target genes ACAA2 and HSD17B12.
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影响因子：
5.6
作者：
Shen Y;Miao N;Xu J;Gan X;Xu D;Zhou L;Xue H;Zhang W;Lu L
通讯作者：
Lu L
影响因子：
5
作者：
Lopez MF;Niu P;Wang L;Vogelsang M;Gaur M;Krastins B;Zhao Y;Smagul A;Nussupbekova A;Akanov AA;Jordan IK;Lunyak VV
通讯作者：
Lunyak VV
影响因子：
21.3
作者：
Celià-Terrassa T;Liu DD;Choudhury A;Hang X;Wei Y;Zamalloa J;Alfaro-Aco R;Chakrabarti R;Jiang YZ;Koh BI;Smith HA;DeCoste C;Li JJ;Shao ZM;Kang Y
通讯作者：
Kang Y
DOI：
10.1016/j.bbrc.2017.01.012
发表时间：
2017-02-05
影响因子：
3.1
作者：
Chen, Ming-Jenn;Cheng, Ya-Min;Shen, Ching-Ju
通讯作者：
Shen, Ching-Ju
DOI：
10.1016/j.jsbmb.2009.05.005
发表时间：
2009-09-01
影响因子：
4.1
作者：
Plourde, Marie;Ferland, Alexandra;Simard, Jacques
通讯作者：
Simard, Jacques