B Cell Subsets in Healthy Children: Reference Values for Evaluation of B Cell Maturation Process in Peripheral Blood
B Cell Subsets in Healthy Children: Reference Values for Evaluation of B Cell Maturation Process in Peripheral Blood
复制标题
DOI：
10.1002/cyto.b.20536
复制
发表时间：
2010-11-01
影响因子：
3.4
通讯作者：
Bernatowska, Ewa
中科院分区：
文献类型：
--
作者：
Piatosa, Barbara;Wolska-Kusnierz, Beata;Bernatowska, Ewa
背景：免疫系统的成熟过程导致各种淋巴细胞群的产生，这些淋巴细胞群具有以特定方式反应的能力，并在细胞表面表达各种标记物。本研究旨在建立儿童和年轻人外周血B淋巴细胞亚群的参考值，以寻找其生理年龄相关变异谱。方法：对292例0 ~ 31岁儿童和青壮年进行B细胞亚群分布检测。基于CD19、IgM、IgD、CD21、CD27和CD38的差异表达，采用四色流式细胞术测定非记忆和记忆、过渡性、幼稚、未成熟边缘区样/仅IgM记忆、切换类型记忆、双阴性、激活和浆细胞样细胞群的相对和绝对大小。在测试组中观察到个体细胞群的相对数量和绝对数量的显著变化。结果：本研究建立了11个年龄组中年龄相关B细胞亚群的参考值，可用于诊断与B细胞成熟过程相关的任何病理，也可用于将实验室结果与影响儿科人群抗体产生的许多缺陷的临床症状相关联的尝试。结论：测定抗体缺乏患者携带的B细胞亚群可能有助于了解疾病的性质并预防其并发症。(c) 2010年国际临床细胞术学会
Background: The process of maturation of the immune system leads to generation of various lymphoid cell populations having the ability to react in specific way and expressing various markers on the cell surface. The study was set up to establish reference values for B lymphocyte subpopulations in peripheral blood of children and young adults to find the spectrum of their physiological age-related variation.Methods: Blood samples were taken from 292 children and young adults aged 0-31 years and tested for distribution of B cell subsets. Relative and absolute sizes of non-memory and memory, transitional, naive, immature marginal zone-like/IgM-only memory, class-switched memory, double negative, activated, and plasmacytoid cell populations were determined by four-color flow cytometry, based on differential expression of CD19, IgM, IgD, CD21, CD27, and CD38. Significant variation both in relative, as well as in absolute numbers of individual cell populations in tested groups was observed.Results: The reference values for age-related B cell subsets in eleven age groups, established as result of this study, may be used in diagnostics of any pathology related to B cell maturation process, as well as in attempts of correlating laboratory results with clinical symptoms of many defects affecting antibody production in pediatric population.Conclusion: Determination of B cell subpopulations carried in patients with antibody deficiencies may help to understand the nature of the disease and prevent its complications. (c) 2010 International Clinical Cytometry Society