A tissue-engineered trachea derived from a framed collagen scaffold, gingival fibroblasts and adipose-derived stem cells.
A tissue-engineered trachea derived from a framed collagen scaffold, gingival fibroblasts and adipose-derived stem cells.
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DOI：
10.1016/j.biomaterials.2010.02.027
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发表时间：
2010-06
期刊：
影响因子：
14
通讯作者：
Ken Kobayashi;Teruhisa Suzuki;Yukio Nomoto;Y. Tada;M. Miyake;A. Hazama;I. Wada;Tatsuo Nakamura;K. Omori
中科院分区：
文献类型：
--
作者：
Ken Kobayashi;Teruhisa Suzuki;Yukio Nomoto;Y. Tada;M. Miyake;A. Hazama;I. Wada;Tatsuo Nakamura;K. Omori
在某些类型的气管疾病中，需要气管切除术。对于气管切除术患者，我们组已在临床上采用胶原海绵配合螺旋聚丙烯支架和网状物制成人工移植物。然而，上皮细胞再生被证实是缓慢的。在本研究中，我们研究了牙龈成纤维细胞（GFBs）和脂肪来源干细胞（ASCs）作为自体移植细胞与人工移植物联合用于气管上皮再生的潜力。在体外与气管上皮细胞共培养中，GFBs刺激上皮细胞分化并重建假分层上皮。ASCs刺激上皮细胞增殖和多层上皮的重建。随后，我们用GFBs和/或ASCs制备了三种生物工程支架，并将其植入大鼠气管缺损。含GFBs的生物工程支架1周后被气管上皮细胞覆盖，移植2周后形成高度纤毛上皮。含ASCs的生物工程支架诱导厚上皮，然后形成含杯状细胞的假分层上皮。此外，GFBs和ASCs的应用对气管上皮的再生具有协同作用，这表明含有GFBs和ASCs的生物工程支架可用于加速气管上皮的再生。
In some types of tracheal disease, tracheal resection is required. For patients with tracheal resection, artificial grafts, made from collagen sponge with a spiral polypropylene stent and mesh, have been clinically used by our group. However, epithelial regeneration was confirmed to be slow. In the present study, we investigated the potential of gingival fibroblasts (GFBs) and adipose-derived stem cells (ASCs) as autologous transplanted cells in combination with artificial graft for tracheal epithelial regeneration. In in vitro co-culturing with tracheal epithelial cells, GFBs stimulated epithelial cell differentiation and reconstruction of a pseudostratified epithelium. ASCs stimulated epithelial cell proliferation and reconstruction of a multi-layered epithelium. Subsequently, we prepared three kinds of bioengineered scaffolds from GFBs and/or ASCs and implanted them into rat tracheal defects. The bioengineered scaffolds containing GFBs were covered with tracheal epithelial cells after 1 week, and highly ciliated epithelium was formed after 2 weeks of transplantation. The bioengineered scaffold containing ASCs induced thick epithelium, and then pseudostratified epithelium containing goblet cells was formed. Furthermore, the application of both GFBs and ASCs had synergistic effects on tracheal epithelial regeneration, suggesting that bioengineered scaffolds containing GFBs and ASCs are useful for hastening tracheal epithelial regeneration.