AIDS-related B-cell lymphoma (ARL): correlation of prognosis with differentiation profiles assessed by immunophenotyping
AIDS-related B-cell lymphoma (ARL): correlation of prognosis with differentiation profiles assessed by immunophenotyping
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DOI：
10.1182/blood-2004-12-4631
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发表时间：
2005-09-01
期刊：
影响因子：
20.3
通讯作者：
Horst, HA
中科院分区：
文献类型：
--
作者：
Hoffmann, C;Tiemann, M;Horst, HA
本研究旨在分析艾滋病相关b细胞淋巴瘤（ARL）的分化谱及其与临床病程的关系。对1989 ~ 2004年89例ARL的石蜡切片进行免疫组织化学染色，检测抗体为CD3、CD10、CD20、CD38、CD138/Syndecan-1 （Syn-1）、多发性骨髓瘤-1 /干扰素调节因子-4 （MUM1/IRF4）、b细胞淋巴瘤蛋白-2 （BCL-2）、BCL-6、潜伏膜蛋白-1 （LMP-1）和Ki-67。CD10和CD20的表达与更好的总生存期相关（OS; P = 0.009和P = 0.04）。CD20的表达与更长的无病生存期相关（DFS; P =.03），而CD138/Syn-1的表达与更短的DFS相关（P =.03）。生发后中心（GC）分化相关免疫表型（BCL-6和CD10阴性，MUM1/IRF4和/或CD138/ Syn-1阳性）患者的OS和DFS较GC分化患者差（P = 0.01）。当对照年龄调整的国际预后指数（1131）、既往艾滋病定义疾病（ADI）和ARL诊断年份时，gc后分化仍与不良OS和DFS显著相关。CD10的表达与保留的免疫能力相关，而CD20在接受高活性抗逆转录病毒治疗的ARL患者中表达较少（P = 0.04）。总之，缺乏CD20或CD10表达和生发后中心特征与ARL较差的预后相关。
This study was undertaken to analyze the differentiation profiles assessed by immunophenotyping in AIDS-related B-cell lymphoma (ARL) and their relation to the clinical course. Paraffin-embedded sections of 89 ARL cases during 1989 to 2004 were stained immunohistochemically with antibodies to CD3, CD10, CD20, CD38, CD138/Syndecan-1 (Syn-1), multiple myeloma-1 /interferon regulatory factor-4 (MUM1/IRF4), B-cell lymphoma protein-2 (BCL-2), BCL-6, latent membrane protein-1 (LMP-1), and Ki-67. Expression of CD10 and CD20 were associated with better overall survival (OS; P =.009 and P =.04, respectively). Expression of CD20 was associated with longer disease-free survival (DFS; P =.03), whereas expression of CD138/Syn-1 was associated with shorter DFS (P =.03). OS and DFS were worse in patients with immunophenotypic profiles related to post-germinal center (GC) differentiation (BCL-6 and CD10 negative, MUM1/IRF4 and/or CD138/ Syn-1 positive) when compared with GC differentiation (P =.01). When controlled for age-adjusted International Prognostic Index (1131), prior AIDS-defining illness (ADI), and year of ARL diagnosis, a post-GC differentiation remained significantly associated with poor OS and DFS. Expression of CD10 was associated with a preserved immunocompetence, whereas CD20 was less frequent in patients developing ARL while on highly active antiretroviral therapy (P =.04). In summary, lack of CD20 or CD10 expression and a post-germinal center signature are associated with a worse prognosis in ARL.