A Highly Selective Pyrophosphate Sensor Based on ESIPT Turn-On in Water
A Highly Selective Pyrophosphate Sensor Based on ESIPT Turn-On in Water
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DOI：
10.1021/ol200054w
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发表时间：
2011-03-18
期刊：
影响因子：
5.2
通讯作者：
Pang, Yi
中科院分区：
文献类型：
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作者：
Chen, Wei-Hua;Xing, Yu;Pang, Yi
焦磷酸盐(PPI)是一个重要的生物靶标。发现双核体系3环2ZN选择性地识别PPI，从而在水中形成pH为7.4时的比率荧光传感器。结合事件通过开启激发态分子内质子转移(ESIPT)而触发了大的荧光响应(类似于100 nm红移)。对聚合酶链式反应实验释放的PPI的检测表明，这种新的探针在生物分析应用中可能是一种有用的工具。
Pyrophosphate (PPi) is a biologically important target. A binuclear system 3 circle 2Zn is found to selectively recognize PPi, leading to a ratiometric fluorescent sensor at pH 7.4 in water. The binding event triggered a large fluorescence response (similar to 100 nm bathochromic shift) by turning on the excited state intramolecular proton transfer (ESIPT). Detection of PPi released from a PCR experiment indicated that this new probe could be a useful tool in bioanalytical applications.