Visualizing changes in brain-derived neurotrophic factor (BDNF) expression using bioluminescence imaging in living mice.
Visualizing changes in brain-derived neurotrophic factor (BDNF) expression using bioluminescence imaging in living mice.
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DOI：
10.1038/s41598-017-05297-x
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发表时间：
2017-07-10
影响因子：
4.6
通讯作者：
Tsuda M
中科院分区：
文献类型：
--
作者：
Fukuchi M;Izumi H;Mori H;Kiyama M;Otsuka S;Maki S;Maehata Y;Tabuchi A;Tsuda M
脑源性神经营养因子（BDNF）在包括记忆巩固在内的多种神经功能的表达中起着重要作用。脑内BDNF水平的改变与神经退行性和神经精神疾病有关。因此，了解BDNF的水平是如何控制的是很重要的。最近，我们产生了一种新的转基因小鼠品系，称为Bdnf-荧光素酶转基因（Bdnf- luc Tg）小鼠，以监测Bdnf表达的变化。在本研究中，我们检测了活体Bdnf-Luc Tg小鼠腹腔注射d-荧光素后的生物发光信号。尽管大脑中Bdnf的表达水平很高，但很难检测到来自大脑区域的信号，可能是因为其难以穿透（短波长）的生物发光。然而，我们可以通过荧光素类似物产生近红外波长的生物发光来检测大脑区域生物发光信号的变化。我们还发现Tg小鼠喂食高脂肪饮食后，体重的增加与腹部的生物发光信号之间存在很强的相关性。这些结果表明，Bdnf表达的变化可以在活体小鼠中可视化，并且Tg小鼠可以成为阐明Bdnf表达在病理生理和生理条件中的作用的有力工具。
Brain-derived neurotrophic factor (BDNF) plays a fundamental role in expressing various neural functions including memory consolidation. Alterations of BDNF levels in the brain are associated with neurodegenerative and neuropsychiatric disorders. Therefore, it is important to understand how levels of BDNF are controlled. Recently we generated a novel transgenic mouse strain, termed the Bdnf-Luciferase transgenic (Bdnf-Luc Tg) mouse, to monitor changes in Bdnf expression. In the present study, we detected the bioluminescence signal from living Bdnf-Luc Tg mice after intraperitoneal administration of d-luciferin. Despite high levels of Bdnf expression in the brain, it was difficult to detect a signal from the brain region, probably because of its poorly penetrable (short-wavelength) bioluminescence. However, we could detect the changes in the bioluminescence signal in the brain region using a luciferin analogue generating a near-infrared wavelength of bioluminescence. We also found a strong correlation between increases in body weight and bioluminescence signal in the abdominal region of Tg mice fed a high-fat diet. These results show that changes in Bdnf expression can be visualized using living mice, and that the Tg mouse could be a powerful tool for clarification of the role of Bdnf expression in pathophysiological and physiological conditions.
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影响因子：
3.7
作者：
Hartman W;Helan M;Smelter D;Sathish V;Thompson M;Pabelick CM;Johnson B;Prakash YS
通讯作者：
Prakash YS
影响因子：
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作者：
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通讯作者：
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作者：
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通讯作者：
Yamada, Kiyofumi