Treating Achilles Tendon Rupture in Rats with Bone-Marrow-Cell Transplantation Therapy
Treating Achilles Tendon Rupture in Rats with Bone-Marrow-Cell Transplantation Therapy
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DOI：
10.2106/jbjs.i.01325
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发表时间：
2010-12-01
影响因子：
5.3
通讯作者：
Iida, Hirokazu
中科院分区：
文献类型：
--
作者：
Okamoto, Naofumi;Kushida, Taketoshi;Iida, Hirokazu
骨髓细胞具有多能性，已被用于多种治疗。我们假设骨髓细胞可能分化为再生肌腱，骨髓细胞中的几种细胞因子可能加速肌腱愈合。短句来源方法9只F344w/Nslc （Fisher）大鼠作为骨髓细胞和间充质干细胞的来源，选取正常跟腱87只，分为3组：BMC组（肌腱周围注射骨髓细胞）、MSC组（肌腱周围注射间充质干细胞）、MSC组（肌腱周围注射间充质干细胞），结果测量包括力学测试、胶原免疫组化、组织学分析和逆转录聚合酶链反应检测转化生长因子- β (tgf - β)和血管内皮生长因子（VEGF）的表达。结果在切口后7天，BMC组的最终衰竭负荷显著大于未治疗组或MSC组（38 N vs 0 9 N或21 N）。p < 0.016)和术后14 d (10 2n vs 6 1n或8 2n，p < 0 016)最终的破坏载荷在BMC组切口后28天(33 8 N)一样正常肌腱(34 8 N) BMC组的人表现出更强的iii型胶原染色七天切口和更强的i型胶原染色后28天比MSC组的表达及在BMC和VEGF组显著增加的价格相比其他组在四天之后切口(及1 6对1 3 0 6,结论全骨髓细胞移植治疗跟腱断裂可能比培养间充质干细胞治疗跟腱断裂更好、更容易。临床意义本研究表明，骨髓细胞移植治疗跟腱断裂可能是一种有前景的治疗方法
Background Bone marrow cells possess multipotentiality and have been used for several treatments We hypothesized that bone marrow cells might differentiate into regenerated tendon and that several cytokines within bone marrow cells might accelerate tendon healing Therefore, we treated Achilles tendon ruptures in a rat model with transplantation of whole bone marrow cellsMethods Nine F344w/Nslc (Fisher) rats were the source of bone marrow cells and mesenchymal stem cells as well as normal Achilles tendons Eighty-seven Fisher rats were used for the experiments The rats were divided into three groups the BMC group (bone marrow cells injected around the tendon), the MSC group (mesenchymal stem cells injected around the tendon), and the non-treated control group (incision only) Outcome measures included mechanical testing, collagen immunohistochemistry, histological analysis, and reverse transcription-polymerase chain reaction to detect expression of transforming growth factor-beta (TGF-beta) and vascular endothelial growth factor (VEGF)Results The ultimate failure load in the BMC group was significantly greater than that in the non-treated or the MSC group at seven days after incision (3 8 N vs 0 9 N or 2 1 N, p < 0 016) and at fourteen days after incision (10 2 N vs 6 1 N or 8 2 N, p < 0 016) The ultimate failure load in the BMC group at twenty-eight days after incision (33 8 N) was the same as that of normal tendon (34 8 N) The BMC group demonstrated stronger staining for type-III collagen at seven days after incision and stronger staining for type-I collagen at twenty-eight days than did the MSC group Expression of TGF-beta and VEGF in the BMC group was significantly increased compared with that in the other groups at four days after incision (TGF-beta 1 6 vs 1 3 or 0 6, p < 0 01, VEGF 1 7 vs 1 1 or 0 9, p < 0 01)Conclusions Transplantation of whole bone marrow cells may be a better and more readily available treatment for Achilles tendon rupture than cultured mesenchymal stem cellsClinical Relevance The present study provides evidence that transplantation of bone marrow cells may be a promising therapy for the treatment of Achilles tendon rupture