Increased expression of aryl hydrocarbon receptor and interleukin 22 in patients with allergic asthma.
Increased expression of aryl hydrocarbon receptor and interleukin 22 in patients with allergic asthma.
复制标题
DOI：
--
复制
发表时间：
2011-09
影响因子：
5
通讯作者：
Jing Zhu;Yong Cao;Kai-yan Li;Zhengyun Wang;P. Zuo;W. Xiong;Yong-jian Xu;S. Xiong
中科院分区：
文献类型：
--
作者：
Jing Zhu;Yong Cao;Kai-yan Li;Zhengyun Wang;P. Zuo;W. Xiong;Yong-jian Xu;S. Xiong
本研究旨在探讨芳烃受体（AhR）和白细胞介素（IL）-22是否参与了过敏性哮喘患者外周血单个核细胞（PBMC）的发病机制，以及它们的表达是否与疾病的严重程度有关。方法过敏性哮喘组18例，哮喘控制组17例，健康对照组12例。收集PBMCs，通过实时定量聚合酶链反应（PCR）检测AhR mRNA。采用酶联免疫吸附试验（ELISA）检测血浆中IL-22蛋白水平。结果过敏性哮喘患者外周血单个核细胞AhR mRNA表达和血浆IL-22蛋白表达均高于正常对照组和正常对照组。血浆IL-22浓度与第一秒用力呼气容积百分比（FEV1%）、FEV 1/用力肺活量百分比（FEV 1/FVC%）呈负相关，与哮喘严重程度评分（ASS）呈正相关。结论AhR和IL-22均参与了过敏性哮喘的发病过程，且IL-22水平与哮喘的严重程度有关。
OBJECTIVES We sought to determine whether the aryl hydrocarbon receptor (AhR) and interleukin (IL)-22 may be involved in the pathogenesis of the peripheral blood mononuclear cells (PBMCs) in allergic asthmatic patients and whether their expression may be related to the severity of the disease. METHODS Blood samples were obtained from each subject with allergic asthma (n =18), controlled asthma (n = 17) and healthy controls (n = 12) respectively. The PBMCs were collected for AhR mRNA detection by real-time quantitative polymerase chain reaction (PCR). The plasma was collected for IL-22 protein detection by enzyme-linked immunosorbent assay (ELISA). RESULTS The expression of AhR mRNA in PBMCs and IL-22 protein in plasma of patients with allergic asthma were higher than those in controlled asthma cases and healthy controls. The plasma concentrations of IL-22 had negative correlation with the predicted percentage of forced expiratory volume in the first second (FEV1%) and the percentage of FEV1 and forced vital capacity (FEV1/FVC%) and it was positively correlated with the asthma severity score (ASS) of the asthmatics. CONCLUSION Our results suggested that both AhR and IL-22 might be involved in the pathogenesis of allergic asthma in human and the level of IL-22 might have some relationship with the severity of the disease.