Organization of immune-responsive medullary projections to the bed nucleus of the stria terminalis, central amygdala, and paraventricular nucleus of the hypothalamus: Evidence for parallel viscerosensory pathways in the rat brain
Organization of immune-responsive medullary projections to the bed nucleus of the stria terminalis, central amygdala, and paraventricular nucleus of the hypothalamus: Evidence for parallel viscerosensory pathways in the rat brain
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DOI：
10.1016/j.brainres.2006.10.084
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发表时间：
2007-01-26
期刊：
影响因子：
2.9
通讯作者：
Goehler, Lisa E.
中科院分区：
文献类型：
--
作者：
Gaykema, Ronald P. A.;Chen, Chiao-Chi;Goehler, Lisa E.
脑干中的免疫反应神经元，主要在孤束核（NTS）和延髓腹外侧（VLM）中，对负责脑介导的宿主防御反应的前脑核团起重要作用。本研究通过逆行追踪和c-Fos免疫组织化学方法研究了大鼠脑干源性上行通路对前脑免疫反应核团的相对贡献。将荧光金离子导入终纹床核（BST）、杏仁中央核（CEA）、下丘脑室旁核（PVN）和脑桥臂旁外侧核（PBL;内脏感觉上行通路的重要组成部分），2周后腹腔注射脂多糖（LPS，0.1mg/kg）或生理盐水。NTS和VLM提供免疫应答输入到所有四个区域，通过直接的，主要是儿茶酚胺能的，投射到PVN，侧BST和CEA，和主要是非儿茶酚胺能的投射到PBL。PBL向BST和CEA提供主要的LPS激活输入。从VLM和NTS到前脑的LPS激活的儿茶酚胺能投射的模式的特征在于VLM输入到PVN的强烈优势，而NTS对BST提供了更大的贡献。这些发现表明，直接和间接途径起源于尾侧脑干，其将免疫相关信息从外周传播到前脑核团，并进行多层次的处理，这可能允许整合大脑对感染的反应。(c)2006年由Elsevier B. V.出版
Immune-responsive neurons in the brainstem, primarily in the nucleus of the solitary tract (NTS) and ventrolateral medulla (VLM), contribute to a significant drive on forebrain nuclei responsible for brain-mediated host defense responses. The current study investigated the relative contribution of brainstem-derived ascending pathways to forebrain immuneresponsive nuclei in the rat by means of retrograde tract tracing and c-Fos immunohistochemistry. Fluorogold was iontophoresed into the bed nucleus of stria terminalis (BST), central nucleus of the amygdala (CEA), paraventricular nucleus of the hypothalamus (PVN), and the pontine lateral parabrachial nucleus (PBL; an important component of ascending viscerosensensory pathways) followed 2 weeks later by intraperitoneal injection of lipopolysaccharide (LPS, 0.1 mg/kg) or saline. The NTS and VLM provide immune-responsive input to all four regions, via direct, predominantly catecholaminergic, projections to the PVN, the lateral BST, and the CEA, and mostly non-catecholaminergic projections to the PBL. The PBL provides a major LPS-activated input to the BST and CEA. The pattern of LPS-activated catecholaminergic projections from the VLM and NTS to the forebrain is characterized by a strong predominance of VLM input to the PVN, whereas the NTS provides a greater contribution to the BST. These findings indicate that direct and indirect pathways originate in the caudal brainstem that propagate immune-related information from the periphery with multiple levels of processing en route to the forebrain nuclei, which may allow for integration of brain responses to infection. (c) 2006 Published by Elsevier B.V.