HU: promoting or counteracting DNA compaction?
HU: promoting or counteracting DNA compaction?
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DOI：
10.1016/s0014-5793(02)03363-x
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发表时间：
2002-10-09
期刊：
影响因子：
3.5
通讯作者：
Goosen, N
中科院分区：
文献类型：
--
作者：
Dame, RT;Goosen, N
HU在大肠杆菌中作为一种参与DNA压实的蛋白质和一种具有调节功能的蛋白质的作用似乎已经得到了牢固的确立。然而，对现有数据的批判性观察表明，对于这种蛋白质的每个提出的作用都不是如此。HU在许多系统中作为调节蛋白或辅助蛋白的作用已经被彻底研究，并且在许多情况下已经在很大程度上被阐明。然而，在其发现近30年后，仍然缺乏令人信服的证据来证明HU在DNA压实中的作用。在这里，我们对现有数据进行了广泛的文献调查，结合新的微观见解，表明HU的作用也可能相反。这种蛋白质可能起着建筑作用，但它不是负责DNA压实，而是参与对抗其他蛋白质（如H-NS）的压实作用。(C) 2002年欧洲生化学会联合会。Elsevier Science B.V.版权所有。
The role of HU in Escherichia coli as both a protein involved in DNA compaction and as a protein with regulatory function seems to be firmly established. However, a critical look at the available data reveals that this is not true for each of the proposed roles of this protein. The role of HU as a regulatory or accessory protein in a number of systems has been thoroughly investigated and in many cases has been largely elucidated. However, almost 30 years after its discovery, convincing evidence for the proposed role of HU in DNA compaction is still lacking. Here we present an extensive literature survey of the available data which, in combination with novel microscopic insights, suggests that the role of HU could be the opposite as well. The protein is likely to play an architectural role, but instead of being responsible for DNA compaction it could be involved in antagonising compaction by other proteins such as H-NS. (C) 2002 Federation of European Biochemical Societies. Published by Elsevier Science B.V. All rights reserved.