Polycistronic lentivirus induced pluripotent stem cells from skin biopsies after long term storage, blood outgrowth endothelial cells and cells from milk teeth
Polycistronic lentivirus induced pluripotent stem cells from skin biopsies after long term storage, blood outgrowth endothelial cells and cells from milk teeth
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DOI：
10.1016/j.diff.2013.01.001
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发表时间：
2013-02-01
期刊：
影响因子：
2.9
通讯作者：
Freund, C.
中科院分区：
文献类型：
--
作者：
Dambrot, C.;van de Pas, S.;Freund, C.
人类诱导多能干细胞（hiPSC）的产生需要收集供体组织，但患者利益具有最高优先级的临床情况可能会损害最终用于重编程的细胞的质量和可用性。在这里，我们比较了（i）在标准生理盐溶液中储存长达两周的皮肤活检，（ii）从新鲜外周血中分离的血液生长内皮细胞（BOEC）和（iii）在正常替换期间丢失的儿童乳牙形成适合于重编程为hiPSC的体细胞培养物的能力。我们使用相同的重编程方法（条件性（FLPe）多顺反子慢病毒）并在相似的条件（同一批病毒、胎牛血清和饲养细胞）下衍生了所有hiPSC系。皮肤成纤维细胞可以重新编程强大，即使在长期活检储存。从幼年牙髓细胞产生hiPSC也具有类似的高效率，但BOEC的效率较低。就活检取样的侵入性、活检储存和重编程效率而言，皮肤成纤维细胞似乎最适合生成hiPSC，但在需要非侵入性程序的情况下（例如，对于儿童和未成年人），来自乳牙的牙髓细胞代表了有价值的替代方案。(C)2013年国际分化学会。Elsevier B.V.出版，保留所有权利。
The generation of human induced pluripotent stem cells (hiPSCs) requires the collection of donor tissue, but clinical circumstances in which the interests of patients have highest priority may compromise the quality and availability of cells that are eventually used for reprogramming. Here we compared (i) skin biopsies stored in standard physiological salt solution for up to two weeks (ii) blood outgrowth endothelial cells (BOECs) isolated from fresh peripheral blood and (iii) children's milk teeth lost during normal replacement for their ability to form somatic cell cultures suitable for reprogramming to hiPSCs. We derived all hiPSC lines using the same reprogramming method (a conditional (FLPe) polycistronic lentivirus) and under similar conditions (same batch of virus, fetal calf serum and feeder cells). Skin fibroblasts could be reprogrammed robustly even after long-term biopsy storage. Generation of hiPSCs from juvenile dental pulp cells gave similar high efficiencies, but that of BOECs was lower. In terms of invasiveness of biopsy sampling, biopsy storage and reprogramming efficiencies skin fibroblasts appeared best for the generation of hiPSCs, but where non-invasive procedures are required (e.g. for children and minors) dental pulp cells from milk teeth represent a valuable alternative. (C) 2013 International Society of Differentiation. Published by Elsevier B.V. All rights reserved.