The tightly regulated copper window in yeast.
The tightly regulated copper window in yeast.
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DOI：
10.1039/c0cc04292g
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发表时间：
2011-02
影响因子：
4.9
通讯作者：
S. Wegner;Fei Sun;N. Hernandez;Chuan He
中科院分区：
文献类型：
--
作者：
S. Wegner;Fei Sun;N. Hernandez;Chuan He
将两种相反的酵母铜调节剂Ace 1和Mac 1转化为两种荧光探针Ace 1-FRET和Mac 1-FRET，其选择性地和灵敏地响应Cu（+）。两种调节剂以及铜储存蛋白Cup 1和Crs 5获得的Cu（+）结合曲线表明，游离铜（i）在酵母内保持在狭窄的窗口中。
The two opposing yeast copper regulators, Ace1 and Mac1, were converted into two fluorescent probes, Ace1-FRET and Mac1-FRET, which selectively and sensitively respond to Cu(+). The Cu(+)-binding curves obtained for both regulators as well as for the copper storage proteins Cup1 and Crs5 show that free copper(i) is maintained in a narrow window inside yeast.