Comparison of the O‐dealkylation of 7‐ethoxycoumarin and the hydroxylation of benzo [a] pyrene in human placenta
Comparison of the O‐dealkylation of 7‐ethoxycoumarin and the hydroxylation of benzo [a] pyrene in human placenta
复制标题
人胎盘中7-乙氧基香豆素的O-脱烷基化与苯并[a]芘的羟基化的比较
DOI：
10.1002/cpt1974164701
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发表时间：
1974
影响因子：
6.7
通讯作者：
A. Conney
中科院分区：
文献类型：
--
作者：
M. Jacobson;W. Levin;P. Poppers;A. Wood;A. Conney
描述了一种高灵敏度的方法，用于测量7 -乙氧基香豆素或香豆素对7 -羟基香豆素的体外代谢。用这种方法测定了12名不吸烟者和12名吸烟妇女胎盘中7 -乙氧基香豆素的O -脱烷基和香豆素的羟基化。虽然从怀孕期间吸烟的妇女获得的每个胎盘中都发现了诱导水平的苯并[a]芘（BP）羟化酶活性，但只有在BP羟化酶活性显著诱导（50至100倍）的胎盘中，才会诱导7 -乙氧基香豆素O -脱烷基酶活性（约2倍）。这些结果表明，只有在BP羟化酶活性显著诱导的个体中，乙基香豆素脱烷基酶活性与人胎盘中BP羟化酶活性相似。在非吸烟者的胎盘匀浆中未观察到香豆素7位羟基化。例如，吸烟的女性。
A highly sensitive method is described for measuring the in vitro metabolism of 7‐ethoxycoumarin or coumarin to 7‐hydroxycoumarin. This method was used to measure the O‐dealkylation of 7‐ethoxycoumarin and the hydroxylation of coumarin by placentas from 12 nonsmokers or from 12 women who smoked cigarettes. Although an induced level of benzo[a]pyrene (BP) hydroxylase activity was found in each placenta obtained from women who smoked cigarettes during pregnancy, 7‐ethoxycoumarin O‐dealkylase activity was induced (about 2 fold) only in placentas whose BP hydroxylase activity was markedly induced (50 to 100 fold). These results indicate that ethoxycoumarin dealkylase activity parallels BP hydroxylase activity in human placenta only in individuals with markedly induced BP hydroxylase activity. No detectable hydroxylation of coumarin in the 7‐position was observed in placental homogenates from nonsmokers 0., from women who smoked cigarettes.