Dual-functional adhesive containing amorphous calcium phosphate nanoparticles and dimethylaminohexadecyl methacrylate promoted enamel remineralization in a biofilm-challenged environment
Dual-functional adhesive containing amorphous calcium phosphate nanoparticles and dimethylaminohexadecyl methacrylate promoted enamel remineralization in a biofilm-challenged environment
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DOI：
10.1016/j.dental.2022.07.003
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发表时间：
2022-08-19
期刊：
影响因子：
5
通讯作者：
Xu, Hockin H. K.
中科院分区：
文献类型：
--
作者：
Fan, Menglin;Li, Meng;Xu, Hockin H. K.
目的：牙釉质、修复体及粘接界面上的致龋生物膜与龋病及复合修复体的失败密切相关。釉质在粘接界面的矿化有利于保护粘接界面，抑制继发龋。本研究旨在评估含有甲基丙烯酸二甲氨基十六烷基酯（DMAHDM）和无定形磷酸钙（NACP）纳米颗粒的粘合剂对生物膜涂层牙釉质初始龋损的再矿化效率。研究方法：用脱矿液浸泡72小时造成人工初始龋损，变形链球菌培养24小时形成致龋生物膜。变种人）。将牙釉质分为对照组、NACP组、DMAHDM组和NACP+DMAHDM组。接下来，样品经历7天循环，每天在BHIS（含有1%蔗糖的脑心浸液肉汤）中4小时，在AS（人工唾液）中20小时。每天检测BHIS的pH值。BHIS和AS中钙、磷浓度也有显著性差异。7天后测量生物膜的活/死染色、菌落形成单位（CFU）计数和乳酸产生。采用显微硬度测试和横向显微X射线照相（TMR）定量评价釉质再矿化效率.
Objective: The cariogenic biofilm on enamel, restoration, and bonding interface is closely related to dental caries and composite restoration failure. Enamel remineralization at ad- hesive interface is conducive to protecting bonding interface and inhibiting secondary caries. This study intended to assess the remineralization efficiency of adhesive with di- methylaminohexadecyl methacrylate (DMAHDM) and nanoparticles of amorphous cal- cium phosphate (NACP) on initial caries lesion of biofilm-coated enamel. Methods: Artificial initial carious lesion was created via 72-hour immersion in deminer- alization solution and cariogenic biofilm was formed after 24-hour culture of Streptococcus mutans (S. mutans). Specimens were then divided into 4 groups: enamel control, enamel treated with NACP, DMAHDM and NACP+DMAHDM respectively. Samples next underwent 7-day cycling, 4 h in BHIS (brain heart infusion broth containing 1 % sucrose) and 20 h in AS (artificial saliva) per day. The pH of BHIS was tested daily. So did the concentration of calcium and phosphate in BHIS and AS. Live/dead staining, colony-forming unit (CFU) count, and lactic acid production of biofilms were measured 7 days later. The enamel re -mineralization efficiency was evaluated by microhardness testing and transverse micro -radiography (TMR) quantitatively.