Dietary arginine supplementation alleviates immune challenge induced by Salmonella enterica serovar Choleraesuis bacterin potentially through the Toll-like receptor 4-myeloid differentiation factor 88 signalling pathway in weaned piglets
Dietary arginine supplementation alleviates immune challenge induced by Salmonella enterica serovar Choleraesuis bacterin potentially through the Toll-like receptor 4-myeloid differentiation factor 88 signalling pathway in weaned piglets
复制标题
日粮补充精氨酸可通过 Toll 样受体 4-骨髓分化因子 88 信号通路减轻断奶仔猪肠沙门氏菌血清型猪霍乱菌苗诱导的免疫挑战
DOI：
10.1017/s0007114511006350
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发表时间：
2012-09-28
影响因子：
3.6
通讯作者：
Yu, Bing
中科院分区：
文献类型：
--
作者：
Chen, Yu;Chen, Daiwen;Yu, Bing
本研究旨在探讨饲粮中添加精氨酸（Arg）是否能通过Toll样受体（TLR）4-髓样分化因子88（Myd 88）信号通路减轻猪霍乱沙门氏菌C500（S. C500）诱导的断奶仔猪免疫攻击。试验选用36头断奶仔猪，随机分为6组，每组6个重复。试验第1周（0-7 d），分别饲喂含0、0.5和1. 0% L-Arg的日粮，每个处理设两组。第8天，猪肌肉注射S。C500或无菌生理盐水。在第8天（注射前）和注射后1、3和10天收集血清样品，在最后一次采血后处死猪以评价组织基因表达。添加0.5%和1.0%精氨酸组仔猪血清精氨酸浓度显著高于对照组（P
The present study evaluated whether dietary arginine (Arg) supplementation could attenuate immune challenge induced by Salmonella enterica serovar Choleraesuis C500 (S.C500) through the Toll-like receptor (TLR) 4-myeloid differentiation factor 88 (Myd88) signalling pathway in weaned piglets. A total of thirty-six weaned pigs were randomly allocated into six groups with six replicates per group. Pigs were subjected to three dietary treatments (namely two groups per treatment) in the first week (0-7 d) and fed with diets containing 0, 0.5 and 1.0% L-Arg, respectively. On day 8, pigs were injected intramuscularly either with S. C500 or sterile saline. Serum samples were collected at day 8 (before injection), and at 1, 3 and 10 d post-injection, pigs were killed for evaluation of tissue gene expression following the last blood collection. Piglets fed the diets with 0.5 or 1.0% Arg supplementation had a higher concentration of serum Arg (P