Identification and expression analysis of conserved microRNAs during short and prolonged chromium stress in rice (Oryza sativa)
Identification and expression analysis of conserved microRNAs during short and prolonged chromium stress in rice (Oryza sativa)
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DOI：
10.1007/s11356-019-06760-0
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发表时间：
2019-12-02
影响因子：
5.8
通讯作者：
Chakrabaroty, Debasis
中科院分区：
文献类型：
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作者：
Dubey, Sonali;Saxena, Sharad;Chakrabaroty, Debasis
MicroRNAs （miRNAs）是调控植物发育和防御反应过程的最重要的表观遗传基因表达调控因子之一。大米作为世界各地的主食，其铬污染严重影响人类健康。虽然一些研究已经确定了水稻mirna在重金属（砷、镉）和热或冷胁迫下的差异响应，但没有关于mirna在Cr胁迫下响应的报道。在本研究中，我们分别从Cr处理和未处理的样品中鉴定出512和568个已知的mirna。表达分析显示，13个保守mirna （miR156、miR159、miR160、miR166、miR169、miR171、miR396、miR397、miR408、miR444、miR1883、miR2877、miR5072）优先上调或下调（P值< 0.05）。靶基因对差异表达mirna的预测及其功能解释表明，mirna通过atp结合盒转运体（ABC转运体）、转录因子、热休克蛋白、生长素反应和金属离子运输在Cr的防御和解毒中发挥重要作用。Real-time PCR分析证实了所选mirna及其推测靶基因的差异表达。总之，我们的研究确定并预测了水稻在Cr胁迫下mirna介导的信号通路调控。
MicroRNAs (miRNAs) are one of the most critical epigenetic regulators of gene expression which modulate a spectrum of development and defence response processes in plants. Chromium (Cr) contamination in rice imposes a serious concern to human health as rice is used as staple food throughout the world. Although several studies have established the differential response of miRNAs in rice during heavy metal (arsenic, cadmium) and heat or cold stress, no report is available about the response of miRNAs during Cr stress. In the present study, we identified 512 and 568 known miRNAs from Cr treated and untreated samples, respectively. Expression analysis revealed that 13 conserved miRNAs (miR156, miR159, miR160, miR166, miR169, miR171, miR396, miR397, miR408, miR444, miR1883, miR2877, miR5072) depicted preferential up- or down-regulation (> 4-fold change; P value < 0.05). Target gene prediction of differentially expressed miRNAs and their functional annotation suggested the important role of miRNAs in defence and detoxification of Cr though ATP-binding cassette transporters (ABC transporters), transcription factors, heat shock proteins, auxin response, and metal ion transport. Real-time PCR analysis validated the differential expression of selected miRNAs and their putative target genes. In conclusion, our study identifies and predicts miRNA-mediated regulation of signalling pathway in rice during Cr stress.