Colorimetric Sensing of Methyl Parathion Using Nanozymatic Activity of Gold Nanoparticles
Colorimetric Sensing of Methyl Parathion Using Nanozymatic Activity of Gold Nanoparticles
复制标题
利用金纳米颗粒的纳米酶活性对甲基对硫磷进行比色传感
DOI：
10.1166/asem.2020.2492
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发表时间：
2020
期刊：
影响因子：
--
通讯作者：
A. Pandey
中科院分区：
文献类型：
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作者：
A. Pandey;Saumya S. Srivastava;Gayatri;Priyan Rai;A. Pandey
在这项研究中，我们使用了新的方法来检测甲基异硫氰酸酯残留，即，通过抑制金纳米颗粒的过氧化物酶样活性的一半。我们通过比色分析研究了金纳米颗粒的过氧化物酶样活性，并优化了pH，
以TMB为底物，研究了温度、孵育时间、不同浓度H_2O_2以及有机磷对它们的酶模拟活性的影响。动力学研究表明，金纳米粒子的催化活性比辣根过氧化物酶在pH 3.5。
过氧化物酶底物TMB（3，3，5，5-四甲基联苯胺）可以通过金纳米颗粒的酶促作用被H2 O2氧化，产生蓝色产物，氧化的TMB。抑制纳米酶的酶活性的原理是
这是由于甲基二价阳离子对TMB-H_2 O_2-Au NPs体系的电子传递机制的阻碍。过氧化物酶的活性选择性地降低与增加的甲基双离子浓度。该传感方法的最低检测限为78.95 nM。本研究可
用于开发敏感和成本效益高的有害农药检测技术。
In this study, we have used new approach for detection of the methyl parathion residues i.e., by inhibiting half of the peroxidase-like activity of gold nanoparticles. We have investigated the peroxidase like activity of gold nanoparticles by colorimetric assay and optimized the pH,
temperature, incubation time and different concentrations of H2O2 by using TMB as substrate as well as organophosphates effect on their enzyme mimetic activity. Kinetic study of gold nanoparticles has shown better catalytic activity than horseradish peroxidase at pH 3.5.
The peroxidase substrate TMB (3,3,5,5-tetramethylbenzidine) can be oxidized by H2O2 by the enzymatic action of the gold nanoparticles resulting in a blue-coloured product, oxidized TMB. The principle involved behind the inhibition of enzymatic activity of nanozyme is
due to hindrance of electron transfer mechanism TMB-H2O2-Au NPs system by the methyl parathion. The peroxidase activity is selectively reduced with increasing methyl parathion concentration. This sensing method has lowest limit of detection of 78.95 nM. This study can
be used for development of sensitive and cost effective technique for sensing of harmful pesticides.