Can microRNA profiling in maternal blood identify women at risk for preterm birth?
Can microRNA profiling in maternal blood identify women at risk for preterm birth?
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DOI：
10.1016/j.ajog.2015.01.023
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发表时间：
2015-06-01
影响因子：
9.8
通讯作者：
Brown, Amy G.
中科院分区：
文献类型：
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作者：
Elovitz, Michal A.;Anton, Lauren;Brown, Amy G.
目的：MicroRNA（miRNAs）是一类高度保守的单链非编码RNA，在基因调控中起着重要作用，现已被鉴定为多种疾病的重要参与者。miRNAs也被证明是可靠和有用的生物标志物，可以识别那些有特定不良后果风险的女性。本研究的目的是确定是否在母体血液中的miRNA谱是不同的妇女谁是注定要有一个早产，与长期，birth.Study设计：一个巢式病例对照研究进行了与母体血清，收集作为一个更大的前瞻性队列的一部分。比较了最终早产妇女（n = 40）与足月分娩妇女（n = 40）之间母体血清中的miRNA表达谱。使用Affyssin GeneChip miRNA Array创建miRNA表达谱。采用微阵列分析和主成分分析对数据进行显著性分析。20%的错误发现率被用来确定最差异表达的miRNAs.RESULTS：在芯片上分析的5640个miRNAs中，有4个miRNAs在病例组和对照组之间存在显著差异。4种miRNAs中有2种为成熟miRNAs。表达的倍数差异为
OBJECTIVE: MicroRNAs (miRNAs), which are highly conserved single-stranded noncoding RNAs that play a crucial role in gene regulation, have now been identified as important players in many diseases states. MiRNAs have also been demonstrated to be reliable and useful biomarkers to identify those women who are at risk for specific adverse outcomes. The objective of this study was to determine whether miRNA profiles in maternal blood are different in women who are destined to have a preterm, compared with a term, birth.STUDY DESIGN: A nested case-control study was performed with maternal serum that was collected as part of a larger prospective cohort. MiRNA expression profiles in maternal serum were compared between women who ultimately had a preterm birth (n = 40) compared with term birth (n = 40). MiRNA expression profiles were created with the use of the Affymetrix GeneChip miRNA Array. The data were analyzed with the significance of analysis of microarrays and principle components analyses. A false discovery rate of 20% was used to determine the most differentially expressed miRNAs.RESULTS: Of the 5640 miRNAs that were analyzed on the array, 4 miRNAs were significantly different between cases and control subjects. Two of the 4 miRNAs were mature miRNAs. The fold difference in expression was