Effect of the non-steroidal anti-inflammatory drugs on the acyl-CoA synthetase activity toward medium-chain, long-chain and polyunsaturated fatty acids in mitochondria of mouse liver and brain
Effect of the non-steroidal anti-inflammatory drugs on the acyl-CoA synthetase activity toward medium-chain, long-chain and polyunsaturated fatty acids in mitochondria of mouse liver and brain
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DOI：
10.3109/14756366.2011.636742
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发表时间：
2013-02-01
影响因子：
5.6
通讯作者：
Masuyama, Teiichi
中科院分区：
文献类型：
--
作者：
Kasuya, Fumiyo;Kazuhiro, Misumi;Masuyama, Teiichi
在小鼠肝和脑线粒体中评价了11种非甾体抗炎药对辛酸、棕榈酸、花生四烯酸和二十二碳六烯酸的酰基辅酶A合成酶活性的影响。试验药物为阿司匹林、水杨酸、二氟尼柳、甲芬那酸、吲哚美辛、依托度酸、布洛芬、酮洛芬、萘普生、洛索洛芬、氟比洛芬。在小鼠肝线粒体中，二氟尼柳和甲芬那酸不仅表现出对辛酸（IC 50 = 78.7和64.7 μ M）的抑制活性，而且还表现出对棕榈酸（IC 50 = 236.5和284.4 μ M）的抑制活性。阿司匹林是一种抑制剂的激活辛酸（IC 50 = 411.0 μ M）。在大脑中，甲芬那酸和二氟尼柳强烈抑制棕榈酰辅酶A的形成（IC 50 = 57.3和114.0 μ M），分别。与肝脏相比，脑中二十二碳六烯酸的活化对二氟尼柳和甲芬那酸的抑制敏感。
Effect of eleven non-steroidal anti-inflammatory drugs on the acyl-CoA synthetase activities toward octanoic, palmitic, arachidonic and docosahexaenoic acids was evaluated in mouse liver and brain mitochondria. The drugs tested were aspirin, salicylic acid, diflunisal, mefenamic acid, indomethacin, etodolac, ibuprofen, ketoprofen, naproxen, loxoprofen, flurbiprofen. In mouse liver mitochondria, diflunisal and mefenamic acid exhibited the inhibitory activities not only for octanoic acid (IC50 = 78.7 and 64.7 mu M) and but also for palmitic acid (IC50 = 236.5 and 284.4 mu M), respectively. Aspirin was an inhibitor for the activation of octanoic acid only (IC50 = 411.0 mu M). In the brain, mefenamic acid and diflunisal inhibited strongly palmitoyl-CoA formation (IC50 = 57.3 and 114.0 mu M), respectively. The activation of docosahexaenoic acid in brain was sensitive to inhibition by diflunisal and mefenamic acid compared with liver.