Comparison of transfection agents in forming complexes with ferumoxides, cell labeling efficiency, and cellular viability.
Comparison of transfection agents in forming complexes with ferumoxides, cell labeling efficiency, and cellular viability.
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DOI：
10.1162/153535004773861697
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发表时间：
2004-01-01
影响因子：
2.8
通讯作者：
Frank, Joseph Alan
中科院分区：
文献类型：
--
作者：
Arbab, Ali Syed;Yocum, Gene Thomus;Frank, Joseph Alan
通过将亚铁氧化物或超顺磁性氧化铁(SPIO)与转染剂(TA)络合，有可能对哺乳动物细胞进行磁性标记。目前还没有系统的研究比较TAS和SPIO的细胞标记效率和活性。本研究探讨了不同剂量的FeS与哺乳动物细胞中不同的TA络合后的毒性和标记效率。使用了不同类别的TA，如聚阳离子胺、树枝状大分子和脂类试剂。在孵育液中，用1~50微克/毫升的TAS剂量测定细胞毒性。通过结合不同量的FeS和不同剂量的TAS来测定铁的掺入效率。脂质体2000的毒性最低(1微克/毫升)，而FuGENE6和低分子量多L赖氨酸的毒性最小。SPIO标记效率与高分子量PLL(388.1 kDa)和SuperFect相似，而FuGENE6和低分子PLL对细胞的标记效率较低。在培养液中，浓度为25~50微克/毫升的FeS与TAS络合，使SPIO有足够的内吞作用进入内体，从而在细胞磁共振成像上检测到细胞。
By complexing ferumoxides or superparamagnetic iron oxide (SPIO) to transfection agents (TAs), it is possible to magnetically label mammalian cells. There has been no systematic study comparing TAs complexed to SPIO as far as cell labeling efficiency and viability. This study investigates the toxicity and labeling efficiency at various doses of FEs complexed to different TAs in mammalian cells. Different classes of TAs were used, such as polycationic amines, dendrimers, and lipid-based agents. Cellular toxicity was measured using doses of TAs from 1 to 50 microg/mL in incubation media. Iron incorporation efficiency was measured by combining various amounts of FEs and different doses of TAs. Lipofectamine2000 showed toxicity at lowest dose (1 microg/mL), whereas FuGENE6 and low molecular weight poly-L-lysine (PLL) showed the least toxicity. SPIO labeling efficiency was similar with high-molecular-weight PLL (388.1 kDa) and superfect, whereas FuGENE6 and low-molecular-weight PLL were inefficient in labeling cells. Concentrations of 25 to 50 microg/mL of FEs complexed to TAs in media resulted in sufficient endocytosis of the SPIO into endosomes to detect cells on cellular magnetic resonance imaging.