Expression analysis of the auxin efflux carrier family in tomato fruit development
Expression analysis of the auxin efflux carrier family in tomato fruit development
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DOI：
10.1007/s00425-010-1211-0
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发表时间：
2010-08-01
期刊：
影响因子：
4.3
通讯作者：
Kanayama, Yoshinori
中科院分区：
文献类型：
--
作者：
Nishio, Sogo;Moriguchi, Ryo;Kanayama, Yoshinori
生长素转运网络在植物发育信号的整合中起着重要作用，它依赖于生长素外排载体PIN基因家族的差异表达。我们克隆了三个番茄PIN（简称SlPIN）cDNA，并研究了它们在果实和其它器官中的表达模式。SlPIN1和SlPIN2的表达在开花后立即在非常年轻的果实中最高，而SlPIN3的表达在果实发育的同一阶段较低。SlPIN2：：GUS在开花期的胚珠和开花后4天的发育中的种子中表达，而SlPIN1：：GUS在整个果实中表达。DR5：：GUS生长素应答报告基因在开花时在果实和花序梗中表达，开花后4天在花序梗中表达量较高。这些研究表明，生长素可能是从年轻的种子运输的SlPIN 1和SlPIN 2和积累在花序梗中，其中SlPIN基因表达低的番茄。对SlPINs在座果中的可能作用进行了讨论。
Auxin transport network, which is important in the integration of plant developmental signals, depends on differential expression of the auxin efflux carrier PIN gene family. We cloned three tomato PIN (referred as SlPIN) cDNAs and examined their expression patterns in fruit and other organs. The expression of SlPIN1 and SlPIN2 was highest in very young fruit immediately after anthesis, whereas the expression of SlPIN3 was low at this same stage of fruit development. SlPIN2::GUS was expressed in ovules at anthesis and in young developing seeds at 4 days after anthesis, while SlPIN1::GUS was expressed in whole fruit. The DR5::GUS auxin-responsive reporter gene was expressed in the fruit and peduncle at anthesis and was higher in the peduncle 4 days after anthesis. These studies suggest that auxin is likely transported from young seeds by SlPIN1 and SlPIN2 and accumulated in peduncles where SlPIN gene expression is low in tomato. The possible role of SlPINs in fruit set was discussed.