Artificial matrix barriers: a diffusion study utilizing dextrans and microspheres.
Artificial matrix barriers: a diffusion study utilizing dextrans and microspheres.
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人工基质屏障：利用葡聚糖和微球的扩散研究。
DOI：
10.1002/ar.1092280104
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发表时间：
1990
期刊：
影响因子：
--
通讯作者：
Hendrix,MJ
中科院分区：
文献类型：
--
作者：
Persky,B;Hendrix,MJ
评价了8种人工基质（AM）限制扩散探针通过的能力。三个AM由间质I型胶原（Col I）组成，仅在厚度上彼此不同。4个AM由复溶基底膜（RBM）涂层聚碳酸酯过滤器（含直径10 μm的孔）组成，仅厚度不同。一个AM由无涂层的10 μm孔聚碳酸酯过滤器组成。扩散探针是不带电荷的异硫氰酸荧光素标记的葡聚糖，分子量为17，900、42，000、71，200和148，900，以及带负电荷的乳胶微球，直径为0.08、0.30和0.95 μm。将探针应用于AM，在37°C下孵育72小时，然后进行分光光度分析。随着分子量或胶原厚度增加（范围7%至0.7%），葡聚糖通过Col I基质的限制越来越多。与Col I基质相比，薄RBM涂层过滤器对葡聚糖的渗透性更高（范围为100%-30%）。Col I基质的微球扩散率（范围3.5%至0）与厚和薄RBM涂层过滤器（范围4%至0）相似。未涂覆的过滤器允许葡聚糖和微球的最大扩散（范围100%至7%）。这些数据表明，本研究中提出的AM将允许直接观察细胞在体外与各种细胞外基质相互作用时的降解和迁移潜力。
Eight artificial matrices (AMs) were evaluated for the ability to restrict the passage of diffusion probes. Three AMs were composed exlusively of interstitial type I collagen (Col I) and differed from each other in thickness only. Four AMs consisted of reconstituted basement membrane (RBM) ‐coated polycarbonate filters (containing 10 μm diameter pores) and also only differed in thickness. One AM consisted of an uncoated 10 μm pore polycarbonate filter. The diffusion probes were uncharged fluorescein isothiocyanate‐labeled dextrans, having molecular weights of 17,900, 42,000, 71,200, and 148,900 and negatively charged latex microspheres, having diameters of 0.08, 0.30, and 0.95 μm. Probes were applied to the AMs, incubated for 72 hr at 37°C, and then analyzed spectrophoto‐metrically. Dextran passage was increasingly restricted for Col I matrices as either molecular weight or collagen thickness increased (range 7% to 0.7%). Thin RBM‐coated filters were more permeable to dextrans (range 100% to 30%) than Col I matrices. The diffusion rate of microspheres for Col I matrices (range 3.5% to 0) was similar to both thick and thin RBM‐coated filters (range 4% to 0). The uncoated filter permitted the most diffusion for both dextrans and microspheres (range 100% to 7%). These data demonstrate that the AMs presented in this study will allow direct observation of the degradative and migratory potential of cells in vitro as they interact with various extracellular matrices.