Multiplex Cytokine Analysis Reveals Elevated Concentration of Interleukin-8 in Glaucomatous Aqueous Humor
Multiplex Cytokine Analysis Reveals Elevated Concentration of Interleukin-8 in Glaucomatous Aqueous Humor
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DOI：
10.1167/iovs.10-5216
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发表时间：
2010-12-01
影响因子：
4.4
通讯作者：
Kuchtey, Rachel W.
中科院分区：
文献类型：
--
作者：
Kuchtey, John;Rezaei, Kasra A.;Kuchtey, Rachel W.
目的.通过比较原发性开角型青光眼（primary open angle glaucoma，POAG）患者和无青光眼白内障患者（对照组）房水（aqueous human，AH）中多种细胞因子的浓度，验证免疫激活在青光眼发生中的假设。使用基于微粒的免疫测定法测量手术期间获得的AH中的细胞因子浓度。IL-8蛋白的局部表达通过人眼的免疫组织化学进行研究。8种细胞因子（IL-1 β、IL-2、IL-4、IL-5、IL-10、IL-12、IFN-γ和TNF-α）低于检测限，2种细胞因子（IL-18和IL-15）检测水平较低或仅在少数患者中检测到。虽然在30名对照患者中的26名（中位数，2.7 pg/mL）和29名POAG患者中的23名（中位数，1.6 pg/mL）中检测到IL-6，但差异无统计学意义。在30名对照患者中的28名（中位数，1.8 pg/mL）和所有29名POAG患者（中位数，4.9 pg/mL）中检测到IL-8。原发性开角型青光眼患者AH中IL-8浓度明显高于正常对照组（P < 0.001）。非对称性青光眼视神经损害患者的双眼中，受累较严重眼的AH中IL-8浓度较高（P < 0.05）。严重视野缺损患者AH中IL-8浓度高于轻度视野缺损患者。IL-8蛋白在人视网膜和视神经中均有表达。POAG患者AH中炎性细胞因子IL-8的浓度显著升高，支持青光眼中发生免疫激活的假设。（Invest Ophthalmol维斯科学。2010;51：6441-6447）DOI：10.1167/iovs.10-5216
PURPOSE. To test the hypothesis that immune activation occurs in glaucoma by comparing concentrations of multiple cytokines in aqueous humor (AH) from patients with primary open angle glaucoma (POAG) and from cataract patients without glaucoma as controls.METHODS. Cytokine concentrations in AH obtained during surgery were measured using microparticle-based immunoassays. Localized expression of IL-8 protein was investigated by immunohistochemistry of human eyes.RESULTS. Eight cytokines (IL-1 beta, IL-2, IL-4, IL-5, IL-10, IL-12, IFN-gamma, and TNF-alpha) were below the limits of detection, and two cytokines (IL-18 and IL-15) were detected at low levels or in only a few patients. Although IL-6 was detected in 26 of 30 control patients (median, 2.7 pg/mL) and in 23 of 29 POAG patients (median, 1.6 pg/mL), the difference was not statistically significant. IL-8 was detected in 28 of 30 control patients (median, 1.8 pg/mL) and in all 29 POAG patients (median, 4.9 pg/mL). The higher IL-8 concentration in the AH of POAG patients was statistically significant (P < 0.001). In pairs of eyes from patients with asymmetric glaucomatous optic nerve damage, IL-8 concentration was higher in the AH of the more severely affected eye (P < 0.05). Patients with severe visual field defects had higher IL-8 concentrations in the AH than did patients with mild visual field defects. IL-8 protein expression was found in human retina and optic nerve.CONCLUSIONS. Concentration of the inflammatory cytokine IL-8 is significantly elevated in the AH of POAG patients, supporting the hypothesis that immune activation occurs in glaucoma. (Invest Ophthalmol Vis Sci. 2010;51:6441-6447) DOI:10.1167/iovs.10-5216