Molecular Characterization of Genome of Mosquito Densovirus Newly Isolated in Yunnan Province
Molecular Characterization of Genome of Mosquito Densovirus Newly Isolated in Yunnan Province
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DOI：
10.3969/j.issn.1005-0507.2011.01.004
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发表时间：
2011-03-01
期刊：
影响因子：
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通讯作者：
Liang Guo-Dong
中科院分区：
文献类型：
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作者：
Feng Yun;Fu Shi-Hong;Liang Guo-Dong
为了解蚊浓核病毒（DNV）的基因组结构，对云南分离的DNV新毒株进行了核苷酸序列测定和分析。引物（DNV 3F、DNV 3R）用于基因组扩增。采用RT-PCR方法扩增片段，直接测序，连接所有核苷酸，获得基因组。应用计算机软件ClustaIX（1.8）、DNAStar、GeneDoc（3.2）对核酸数据进行分析，推导氨基酸序列，并进行同源性分析。从三带喙库蚊（Culex tritaeniorhynchus）、中华按蚊（Anopheles sinensis）、库蚊（Cx. pseudovishnui（1个分离物）、Cx.环带（1株分离株）和Cx。致倦库蚊（pipiens quinquefasciatus）1个分离物。结果表明，新分离株与国外分离株的核苷酸序列同源性为99.9%~ 100%，氨基酸序列同源性为99.7%~ 100%。NS 1和NS 2基因组序列分析表明，云南分离到的14株浓核病毒与国内分离到的浓核病毒同源性最高。
To provide information for the genomic structure of mosquito densovirus (DNV), nucleotide of DNVs new strain isolated in Yunnan was sequenced and analyzed. Primers (DNV3F, DNV3R) were used for genome amplification. RT-PCR was performed to amplify the fragments, which were sequenced directly and all the nucleotides were connected to acquire the genome. The computer software was used to analyze the nucleic acid data, deduce the amino acid sequence and phylogeny including ClustaIX (1.8), DNAStar, GeneDoc (3.2). The 14 DNVs were isolated from Culex tritaeniorhynchus (10 isolates), Anopheles sinensis (1 isolate), Cx. pseudovishnui (1 isolate), Cx. annulus (1 isolate) and Cx. pipiens quinquefasciatus (1 isolate) of Yunnan, China. The results revealed that the new isolates were with high homology in nucleotide sequence (99.9% to 100%), and in amino acid sequence (99.7% to 100%), to the strains isolated from other provinces. Analysis based on the NS1 and NS2 genome sequences showed that the 14 new isolates of densovirus from Yunnan were with the closest homology to those formerly isolated from China.