Production of an extracellular polysaccharide bioflocculant by Klebsiella pneumoniae
Production of an extracellular polysaccharide bioflocculant by Klebsiella pneumoniae
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DOI：
10.1271/bbb.63.2064
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发表时间：
1999-12-01
影响因子：
1.6
通讯作者：
Kurane, R
中科院分区：
文献类型：
--
作者：
Nakata, K;Kurane, R
肺炎克雷伯氏菌H12在乙醇培养基中产生一种新鉴定的胞外多糖，产量为3.0 g/l。多糖的摩尔组成为半乳糖56.04%，葡萄糖25.92%，葡萄糖醛酸10.92%，甘露糖3.71%，葡萄糖醛酸3.37%。添加0.5%~ 1.5%NaCl可提高产量。在300-ppm的CaCl 2溶液中，多糖以1 ppm的浓度在悬浮液中与高岭土絮凝。几乎所有的细菌种细胞聚集在多糖溶液中。高岭土的絮凝能力随pH值和共存的阳离子和阴离子种类的浓度而变化。多糖絮凝剂可参与体内细菌聚集或粘附宿主生物体。
Klebsiella pneumoniae H12 produced a newly identified extracellular polysaccharide in an ethanol medium with a yield of 3.0 g/l. The molar composition of the polysaccharide was 56.04% galactose, 25.92% glucose, 10.92% glucuronic acid, 3.71% mannose, and 3.37% glucuronic acid. The addition of 0.5%-1.5% NaCl increased production. The polysaccharide flocculated with kaolin clay in suspension at the concentration of 1 ppm in a 300-ppm solution of CaCl2. Almost all bacterial species cells aggregated in the polysaccharide solution. The ability to flocculate with kaolin clay changed with the pH and with the concentrations of coexisting cation and anion species. The polysaccharide flocculant mag participate in in vivo bacterial aggregation or adherence to host organisms.