Identification of autoantibodies expressed in acquired aplastic anaemia
Identification of autoantibodies expressed in acquired aplastic anaemia
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DOI：
10.1111/bjh.12116
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发表时间：
2013-02-01
影响因子：
6.5
通讯作者：
Watanabe, Naoki
中科院分区：
文献类型：
--
作者：
Goto, Maki;Kuribayashi, Kageaki;Watanabe, Naoki
获得性再生障碍性贫血（aAA）被认为是一种自身免疫性疾病;然而，涉及的自身抗原和靶细胞仍然难以捉摸。使用造血细胞系K562和骨髓基质细胞系hTS-5检测自身抗体及其靶细胞的表达;分别有43.5%和21.7%的aAA表达针对K562和hTS-5细胞的自身抗体。通过重组cDNA表达克隆进行抗原血清学鉴定，鉴定自身抗原。本研究表明造血细胞是aAA免疫异常的靶细胞。这些自身抗体可用于区分与免疫异常相关的患者和骨髓衰竭综合征。
Acquired aplastic anaemia (aAA) is recognized as an autoimmune disorder; however, the autoantigens and target cells involved remain elusive. Expression of autoantibodies and their target cells were examined using the haematopoietic cell line K562 and bone marrow stromal cell line hTS-5; 43.5% and 21.7% of aAA expressed autoantibody against K562 and hTS-5 cells, respectively. The autoantigens were identified by serological identification of antigens through recombinant cDNA expression cloning. This study indicates that haematopoietic cells are the targets of immune abnormality in aAA. These autoantibodies may be utilized to distinguish patients associated with immune abnormality from bone marrow failure syndrome.