Direct evaluation of acidification by rat proximal tubule: role of carbonic anhydrase.
Direct evaluation of acidification by rat proximal tubule: role of carbonic anhydrase.
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大鼠近曲小管酸化的直接评估：碳酸酐酶的作用。
DOI：
10.1152/ajprenal.1980.238.5.f372
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发表时间：
1980
期刊：
影响因子：
--
通讯作者：
N. Carter
中科院分区：
文献类型：
--
作者：
M. Lucci;L. Pucacco;T. Dubose;J. Kokko;N. Carter
方法一般技术。实验在体重为170-270 g的雄性Sprague-Dawley大鼠上进行。通过腹膜内注射Inactin（100 mg/kg）麻醉大鼠，并通过加热台将其体温保持在37 ℃。进行气管切开术，监测股动脉血压，并以1.5 ml/h的速度将林格氏碳酸氢盐溶液维持输注至股静脉。使用Corning血气分析仪在0.25ml样品上测量动脉血pH和PC 0。使用侧腹切口暴露左肾，固定在Lucite杯中，并用3%琼脂盐水包围。肾脏表面用5%CO2/95%O2平衡的矿物油浸泡并加热至37 ℃.通过静脉注射0.02 ml 10%丽丝胺绿色测量近端小管通过时间。仅使用通过时间小于12 s的肾脏。浅表肾单位近端小管灌注
METHODSGeneral techniques. Experiments were performed on male Sprague-Dawley rats weighing 170-270 g. The rats were anesthetized with an intraperitoneal injection of Inactin (100 mg/kg), and their body temperature was maintained at 37 C by a heated table. A tracheostomy was performed, femoral arterial blood pressure monitored, and maintenance infusion of Ringer bicarbonate solution infused into the femoral vein at 1.5 ml/h. Arterial blood pH and PCO~ were measured on 0.25 ml samples using a Corning blood gas analyzer. The left kidney was exposed using a flank incision, immobilized in a Lucite cup, and surrounded with 3% agar in saline. The kidney surface was bathed with mineral oil equilibrated with 5% CO,/95% 02 and heated to 37 C. Proximal tubule transit times were measured by injecting 0.02 ml of 10% lissamine green intravenously. Only those kidneys whose transit times were less than 12 s were used. Proximal tubules of superficial nephrons were perfused