Induction of bone morphogenetic protein-2 by interleukin-1 in human fibroblasts
Induction of bone morphogenetic protein-2 by interleukin-1 in human fibroblasts
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DOI：
10.1006/bbrc.1998.8988
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发表时间：
1998-07-30
影响因子：
3.1
通讯作者：
Thornton, RD
中科院分区：
文献类型：
--
作者：
Fowler, MJ;Neff, MS;Thornton, RD
风湿性关节炎和牙周炎是与组织破坏相关的慢性炎性疾病，其部分由升高水平的细胞因子（例如，白细胞介素-1和肿瘤坏死因子）。差异筛选的人滑膜成纤维细胞cDNA文库的白细胞介素-1诱导的基因揭示了一个克隆相同的基因编码人骨形态发生蛋白-a。人滑膜成纤维细胞mRNA的北方印迹分析证实了白细胞介素1存在下骨形态发生蛋白2的上调。利用特异性抗体，在白细胞介素-1的存在下，滑膜成纤维细胞的条件培养基中的骨形态发生蛋白-2蛋白水平也上调。这是第一份关于滑膜成纤维细胞产生骨形态发生蛋白-a的报告，也是第一份关于其对白细胞介素-1的上调反应的报告。然而，白细胞介素-1并没有诱导骨形态发生蛋白-α mRNA在人牙龈成纤维细胞。(C)北京：科学出版社.
Rheumatoid arthritis and periodontitis are chronic inflammatory diseases associated with tissue destruction that is mediated in part by elevated levels of cytokines (e.g., interleukin-1 and tumor necrosis factor). Differential screening of a human synovial fibroblast cDNA library for interleukin-1 induced genes revealed a clone identical to the gene encoding human bone morphogenetic protein-a. Northern blot analysis of human synovial fibroblast mRNA confirmed up-regulation of bone morphogenetic protein-2 in the presence of interleukin-1. Utilizing a specific antibody, levels of bone morphogenetic protein-2 protein in conditioned medium from synovial fibroblasts were also up-regulated in the presence of interleukin-1. This is the first report of the production of bone morphogenetic protein-a by synovial fibroblasts, and the first report of its up-regulation in response to interleukin-1. However, interleukin-1 did not induce bone morphogenetic protein-a mRNA in human gingival fibroblasts. (C) 1998 Academic Press.