Telomere dynamics during replicative senescence are not directly modulated by conditions of oxidative stress in IMR90 fibroblast cells
Telomere dynamics during replicative senescence are not directly modulated by conditions of oxidative stress in IMR90 fibroblast cells
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DOI：
10.1007/s10522-009-9216-4
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发表时间：
2009-12-01
期刊：
影响因子：
4.5
通讯作者：
Baird, Duncan M.
中科院分区：
文献类型：
--
作者：
Britt-Compton, Bethan;Wyllie, Fiona;Baird, Duncan M.
许多细胞类型的复制寿命是由起始细胞群中端粒的长度决定的。与氧化应激水平较低的条件相比，在20%氧气条件下，IMR90细胞的复制寿命较短。我们试图解决端粒动力学在决定IMR90细胞复制寿命中的作用。我们分析了在3和20%氧气条件下平行培养的无性系群体。我们观察到，在衰老时，端粒长度在3%的氧气中较短，这与寿命延长成正比。我们观察到，在两种培养条件下，端粒侵蚀率没有可检测到的差异，然而，随着细胞接近衰老，培养物的生长速度减慢，端粒侵蚀率相应增加。我们得出结论，在20%的氧气条件下，IMR90的衰老是端粒无关的，但在3%的氧气条件下，端粒依赖。
The replicative lifespan of many cell types is determined by the length of telomeres in the initiating cell population. In 20% oxygen, IMR90 cells have a shorter replicative lifespan compared to that achieved in conditions that lower the levels of oxidative stress. We sought to address the role of telomere dynamics in determining the replicative lifespan of IMR90 cells. We analysed clonal populations cultured in parallel in 3 and 20% oxygen. We observed that, at senescence, telomere length was shorter in 3% oxygen and this was proportional to the lifespan extension. We observed no detectable difference in the rate of telomere erosion in the two culture conditions, however as the cells approached senescence the growth rate of the cultures slowed with a commensurate increase in the rate of telomere erosion. We conclude that, in 20% oxygen senescence of IMR90 is telomere-independent, but telomere-dependent in 3% oxygen.