Analysis of factors affecting the ratio of microcystin to chlorophyll‐a in cyanobacterial blooms using real‐time polymerase chain reaction
Analysis of factors affecting the ratio of microcystin to chlorophyll‐a in cyanobacterial blooms using real‐time polymerase chain reaction
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DOI：
10.1002/tox.20525
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发表时间：
2011-02
影响因子：
4.5
通讯作者：
J. Ha;T. Hidaka;H. Tsuno
中科院分区：
文献类型：
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作者：
J. Ha;T. Hidaka;H. Tsuno
叶绿素a浓度已被用作水体中微囊藻毒素水平的一个指标。本研究检测了日本一个鱼塘中微囊藻毒素与叶绿素a的比值。在为期六个月的实地调查中，这一比例在空间和时间上都有所变化，这与其他研究人员的报告一致。本研究采用实时荧光定量PCR技术对微囊藻毒素合成酶（microcystin synthetase, mcy） A基因进行定量分析，探讨该比值变化的原因，以观察产微囊藻毒素的蓝藻和微囊藻菌株在天然蓝藻华中的生长情况。real - time PCR的应用证实了毒力与毒力之间的关系。微囊藻毒素与叶绿素a的比值受有毒水华持续时间和携带mcy a基因的产生微囊藻毒素的蓝藻数量的综合影响，尤其是有毒的微囊藻菌株。当产生微囊藻毒素的高浓度微囊藻聚集在低生长速率的静止状态时，蓝藻华产生更多的微囊藻毒素。多变的毒性花需要反映在准确和有效的警报系统有毒的蓝藻和蓝藻毒素。©2009 Wiley期刊公司环境科学与技术，2011(6):391 - 398。
Chlorophyll‐a concentration has been used as an indicator to estimate microcystin levels in water bodies. This study examined the microcystin to chlorophyll‐a ratio in a fishpond in Japan. The ratio varied spatially and temporally during the six‐month field survey, which is consistent with reports by other researchers. We investigated the causes of the variability of the ratio by quantifying microcystin synthetase (mcy) A gene with real‐time PCR, so as to observe the growth of microcystin‐producing cyanobacteria and Microcystis strains in natural cyanobacterial blooms. The application of real‐time PCR enabled corroboration of the relationship between the toxigenicity and the toxicity of the blooms. The microcystin to chlorophyll‐a ratio was influenced by the combined effects of the durability of the toxic bloom, and the quantity of microcystin‐producing cyanobacteria carrying the mcy A gene, especially toxic Microcystis strains. Cyanobacterial blooms produced more microcystin when high concentrations of microcystin‐producing Microcystis aggregated in a stationary state with low growth rates. The variable toxicity of blooms needs to be reflected in accurate and efficient alert systems for toxic cyanobacteria and cyanotoxins. © 2009 Wiley Periodicals, Inc. Environ Toxicol 26: 21–28, 2011.