Zebrafish as a Model to Study the Role of Peroxisome Proliferating-Activated Receptors in Adipogenesis and Obesity.
Zebrafish as a Model to Study the Role of Peroxisome Proliferating-Activated Receptors in Adipogenesis and Obesity.
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DOI：
10.1155/2015/358029
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发表时间：
2015
期刊：
影响因子：
2.9
通讯作者：
Legler J
中科院分区：
文献类型：
--
作者：
Den Broeder MJ;Kopylova VA;Kamminga LM;Legler J
过氧化物酶体激活受体（PPARs）PPARA和PPARD是脂质代谢的调节剂，在通过脂质分解的能量释放中起重要作用，而PPARG在脂质储存和脂肪形成中起关键作用。本文综述了PPARs在脂质代谢、脂肪形成和肥胖中的作用，并评价了斑马鱼作为研究PPARs功能的新兴脊椎动物模型的价值。斑马鱼是研究人类疾病的合适模型，包括肥胖和相关代谢疾病，作为哺乳动物和鱼类之间保守的脂肪形成和脂质代谢的重要途径。本文综述了PPARs在斑马鱼脂肪形成中的作用，并重点介绍了PPARs在斑马鱼脂肪形成中的功能。使用计算机模拟分析，我们证实了斑马鱼基因组中存在五种PPARs（ppara，pparab，pparda，ppardb和pparg），与人类和小鼠PPARs具有67-74%的同一性。在发育过程中，pparda/B旁系同源物和pparg在鱼鳔和胰腺周围显示mRNA表达，这是脂肪细胞首先发育的区域，而pparg在受精后15天（dpf）在脂肪细胞中可检测到。该综述表明，斑马鱼是研究PPARs在脂肪形成和肥胖中的特定功能的一个有前途的模型。
The Peroxisome Proliferator-Activated Receptors (PPARs) PPARA and PPARD are regulators of lipid metabolism with important roles in energy release through lipid breakdown, while PPARG plays a key role in lipid storage and adipogenesis. The aim of this review is to describe the role of PPARs in lipid metabolism, adipogenesis, and obesity and evaluate the zebrafish as an emerging vertebrate model to study the function of PPARs. Zebrafish are an appropriate model to study human diseases, including obesity and related metabolic diseases, as pathways important for adipogenesis and lipid metabolism which are conserved between mammals and fish. This review synthesizes knowledge on the role of PPARs in zebrafish and focuses on the putative function of PPARs in zebrafish adipogenesis. Using in silico analysis, we confirm the presence of five PPARs (pparaa, pparab, pparda, ppardb, and pparg) in the zebrafish genome with 67–74% identity to human and mouse PPARs. During development, pparda/b paralogs and pparg show mRNA expression around the swim bladder and pancreas, the region where adipocytes first develop, whereas pparg is detectable in adipocytes at 15 days post fertilization (dpf). This review indicates that the zebrafish is a promising model to investigate the specific functions of PPARs in adipogenesis and obesity.