FREQUENCY OF THE STAGES IN THE CYCLE OF THE SEMINIFEROUS EPITHELIUM IN THE RAT
FREQUENCY OF THE STAGES IN THE CYCLE OF THE SEMINIFEROUS EPITHELIUM IN THE RAT
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DOI：
10.1095/biolreprod43.3.517
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发表时间：
1990-09-01
影响因子：
3.6
通讯作者：
KEEN, JE
中科院分区：
文献类型：
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作者：
HESS, RA;SCHAEFFER, DJ;KEEN, JE
当生精上皮周期中阶段频率的变化作为评估给药对精子发生影响的标准时，本研究确定了每个睾丸横截面待计数的小管的最佳数量以及每个给药组的动物数量。从15只Sprague-Dawley大鼠的睾丸横截面收集了9672个观察和分期的小管的数据库。发现动物之间I、II、IV、VI、VIII和XIII期的频率存在显著差异。使用计算机模拟从观察数据中随机选择动物和小管数量的不同组合。从每个模拟实验的阶段频率平均值进行了统计学比较，观察到的平均频率。分析了使用所有14个阶段数据的模型。得出以下结论：a）推荐至少200个小管横截面/睾丸用于估计阶段频率; B）对于固定数量的小管评分，在减少方差方面，取样动物的数量比每只动物的小管数量更重要; c）为了检测平均值的2个标准差，误差率为2%，并检查200个小管/睾丸，在评估所有14个阶段时，每组必须使用至少12只动物; d）当使用每组10只动物检查各个阶段时，只有阶段VII具有80%或更大的检验效力（α）。= 0.05）检测频率差异; e）建议3-4组合并阶段，以提高检测治疗差异的把握度。
This study determined the optimum number of tubules to be counted per testis cross section, and the number of animals per treatment group, when changes in stage frequencies in the cycle of the seminiferous epithelium are criteria for assessing effects of treatment on spermatogenesis. A data base of 9 672 observed and staged tubules was collected from testicular cross sections of 15 Sprague-Dawley rats. A significant variation between animals was found for the frequencies of Stages I, II, IV, VI, VIII, and XIII. Computer simulation was used to randomly select different combinations of animal and tubule numbers from the observed data. Stage frequency means from each simulation experiment were compared statistically to observed mean frequencies. A model that used data from all 14 stages was analyzed. The following conclusions were made: a) a minimum of 200 tubule cross sections/testis is recommended for estimating stage frequencies; b) for a fixed number of tubule scored, the number of animals sampled is more important than than the number of tubules per animal in reducing variance; c) to detect of 2 standard deviations form the mean with a 2% error rate and examining 200 tubules/testis, at least 12 animals must be used per group when assessing all 14 stages; d) when individual stages are examined using 10 animals per group, only Stage VII has 80% or greater power of test (.alpha. = 0.05) to detect a frequency difference; e) pooling stages in 3-4 groups is recommended to improve the power of detecting a treatment difference.