Effect of certain bioactive plant extracts on clinical isolates of β-lactamase producing methicillin resistant Staphylococcus aureus
Effect of certain bioactive plant extracts on clinical isolates of β-lactamase producing methicillin resistant Staphylococcus aureus
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DOI：
10.1002/jobm.200410355
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发表时间：
2005-01-01
影响因子：
3.1
通讯作者：
Ahmad, I
中科院分区：
文献类型：
--
作者：
Aqil, F;Khan, MSA;Ahmad, I
研究人员对来自 10 种以抗菌活性而闻名的印度药用植物的乙醇提取物和部分馏分抑制临床分离的产生 P-内酰胺酶的耐甲氧西林金黄色葡萄球菌 (MRSA) 和对甲氧西林敏感的金黄色葡萄球菌 (MSSA) 的能力进行了研究。还评估了植物提取物与某些抗生素的协同相互作用。 MRSA测试菌株被发现具有多重耐药性，并且对常见的内酰胺抗生素也表现出高水平的耐药性。这些菌株产生β-内酰胺酶，可水解一种或其他经过测试的β-内酰胺抗生素。茶树（叶）、凤凰木（花）、Holarrina antidysehterica（树皮）、Lawsonia inermis（叶）、石榴（果皮）、榄仁树（果实）和榄仁树（果实）的植物提取物对所有测试细菌均表现出广谱抗菌活性，抑菌圈大小为 11 毫米至 27 转。圣罗勒叶提取物对三种 MRSA 菌株表现出更好的活性。另一方面，大蒜（球茎）和柑橘（果皮）的提取物对 MRSA 菌株表现出很少或没有活性。通过试管稀释法以最低抑菌浓度（MIC）测定粗提物的抗菌效力。植物提取物针对测试细菌的 MIC 值范围为 1.3 至 8.2 mg/ml。此外，将石榴和凤凰木的提取物在苯、丙酮和甲醇中分馏。在丙酮和甲醇馏分中观察到抗菌活性。检测了茶树、无刺花、石榴、榄仁树和榄仁树粗提物与四环素的体外协同相互作用。此外，茶树提取物还显示出与氨苄西林的协同作用。上述提取物的薄层色谱分析表明存在主要植物化合物，如生物碱、糖苷、黄酮类、酚类和皂苷。 TLC-生物自显影表明酚类和类黄酮是主要的活性化合物。
Ethanolic extracts and some fractions from 10 Indian medicinal plants, known for antibacterial activity, were investigated for their ability to inhibit clinical isolates of P-lactamase producing methicillin-resistant Staphylococcus aureus (MRSA) and methicillin-sensitive S. aureus (MSSA). Synergistic interaction of plant extracts with certain antibiotics was also evaluated. The MRSA test strains were found to be multi-drug resistant and also exhibited high level of resistance to common lactam antibiotics. These strains produced beta-lactamases, which hydrolyze one or other beta-lactam antibiotics, tested. The extract of the plants from Camellia sinensis (leaves), Delonix regia (flowers), Holarrhena antidysehterica (bark), Lawsonia inermis (leaves), Punica granatum (rind), Terminalia chebula (fruits) and Terminalia belerica (fruits) showed a broad-spectrum of antibacterial activity with an inhibition zone size of 11 mm to 27 turn, against all the test bacteria. The extracts from the leaves of Ocimum sanctum showed better activity against the three MRSA strains. On the other hand, extracts from Allium sativum (bulb) and Citrus sinensis (rind) exhibited little or no activity, against MRSA strains. The antibacterial potency of crude extracts was determined in terms of minimum inhibitory concentration (MIC) by the tube dilution method. MIC values, of the plant extracts, ranged from 1.3 to 8.2 mg/ml, against the test bacteria. Further, the extracts from Punica granatum and Delonix regia were fractionated in benzene, acetone and methanol. Antibacterial activity was observed in acetone as well as in the methanol fractions. In vitro synergistic interaction of crude extracts from Camellia sinensis, Lawsonia inermis, Punica granatum, Terminalia chebula and Terminalia belerica was detected with tetracycline. Moreover, the extract from Camellia sinensis also showed synergism with ampicillin.TLC of the above extracts revealed the presence of major phytocompounds, like alkaloids, glycosides, flavonoids, phenols and saponins. TLC-bioautography indicated phenols and flavonoids as major active compounds.