An Acidic Matrix Protein, Pif, Is a Key Macromolecule for Nacre Formation
An Acidic Matrix Protein, Pif, Is a Key Macromolecule for Nacre Formation
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DOI：
10.1126/science.1173793
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发表时间：
2009-09-11
期刊：
影响因子：
56.9
通讯作者：
Nagasawa, Hiromichi
中科院分区：
文献类型：
--
作者：
Suzuki, Michio;Saruwatari, Kazuko;Nagasawa, Hiromichi
软体动物的壳是由碳酸钙晶体和有机基质组成的硬组织。贝壳的珍珠层的特征在于堆叠的隔室结构，每个隔室中具有均匀取向的文石晶体c轴。利用碳酸钙结合试验，我们确定了一种酸性基质蛋白，PIF，在珍珠牡蛎合浦珠母贝，特异性结合文石晶体。Pif互补DNA（cDNA）编码前体蛋白，该前体蛋白被后切割以产生Pif 97和Pif 80。来自免疫定位、使用RNA干扰的敲除实验和体外碳酸钙结晶研究的结果强烈表明Pif调节珍珠层形成。
The mollusk shell is a hard tissue consisting of calcium carbonate crystals and an organic matrix. The nacre of the shell is characterized by a stacked compartment structure with a uniformly oriented c axis of aragonite crystals in each compartment. Using a calcium carbonate-binding assay, we identified an acidic matrix protein, Pif, in the pearl oyster Pinctada fucata that specifically binds to aragonite crystals. The Pif complementary DNA (cDNA) encoded a precursor protein, which was posttranslationally cleaved to produce Pif 97 and Pif 80. The results from immunolocalization, a knockdown experiment that used RNA interference, and in vitro calcium carbonate crystallization studies strongly indicate that Pif regulates nacre formation.