Microsatellites in plants: a new class of molecular markers

Microsatellites in plants: a new class of molecular markers
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DOI:
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发表时间:
2002
期刊:
影响因子:
1
通讯作者:
P. Gupta;H. Balyan;P. C. Sharma
P. Gupta;H. Balyan;P. C. Sharma
中科院分区:
综合性期刊4区
文献类型:
--
作者:
P. Gupta;H. Balyan;P. C. Sharma

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综述了高等植物微卫星(又称简单重复序列或短串联重复序列)的研究现状。对不同植物材料的DNA序列数据库中检测或检索的不同微卫星的发生、分布和多态性水平的结果进行了列表和简要描述。每个SSR的基因座数量和每个基因座的等位基因数量的信息,如果可用的话,也包括在内。本文简要地讨论了微卫星标记的发展方法,所取得的成就以及微卫星标记在分离群体的选择和诊断、基因渐渗过程中的基因组选择等方面应用的可能性(在回交程序中)、基因组作图、基因标记、品种鉴定、种质特性、遗传相关性的估计等也已经被审查和批判性地讨论,微卫星标记的加入,其他可用的分子标记的剧目,应证明是非常有用的,在各种研究领域涉及植物系统。
The present status of research on microsatellites, also called simple sequence repeats (SSRs) or short tandem repeats (STRs) in higher plants has been reviewed. Results on the occurrence, distribution and the level of polymorphism of different microsatellites examined or searched from DNA sequence databases for different plant materials have been tabulated and briefly described. Information on number of loci for each SSR and the number of alleles at each locus, wherever available, has also been included. Methods used for developing microsatellite markers have been briefly discussed, Achievements made and the future possibilities for the use of microsatellites in areas like selection and diagnostics in segregating populations, genome selection during gene introgression (in backcross programmes), genome mapping, gene tagging, cultivar identification, germplasm characterization, estimation of genetic relatedness, etc, have also been reviewed and critically discussed, The addition of microsatellite markers to the repertoire of other available molecular markers should prove very useful in a variety of research areas involving plant systems.