Different behavior of two Trypanosoma cruzi major clones:: Transmission and circulation in young Bolivian patients
Different behavior of two Trypanosoma cruzi major clones:: Transmission and circulation in young Bolivian patients
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DOI：
10.1006/expr.1998.4295
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发表时间：
1998-07-01
影响因子：
2.1
通讯作者：
Tibayrenc, M
中科院分区：
文献类型：
--
作者：
Brenière, SF;Bosseno, MF;Tibayrenc, M
两个克氏锥虫大克隆的不同行为：玻利维亚年轻患者的传播和循环。中华寄生虫病杂志（英文版）；首先通过与大量克氏锥虫种群的杂交分析，分析了两种广泛分布的克氏锥虫克隆基因型（20和39）的特异性，这些种群的多基因研究依赖于MLEE和RAPD。然后，通过聚合酶链反应和克隆特异性DNA杂交的结合，在玻利维亚流行地区的乍得儿童的血液中检测到这些克隆。这些克隆体在患者中的分布与在同一地区的载体（感染三角瘤）中观察到的分布有显著不同。在载体中，克隆体20和39的发现频率相当（分别为0.69和0.67），而在患者中，克隆体20和混合感染的频率较低。马达加斯加人群可分为急性感染和高于寄生虫病公认标准的潜伏感染（直接显微镜检查）。结果表明，在这两种克隆体的传播中存在有限的选择，并且儿童患者的免疫系统对克隆体20寄生虫病有进一步的严格控制。(C) 1998学术出版社。
Different behavior of two Trypanosoma cruzi major clones: Transmission and circulation in young Bolivian patients. Experimental Parasitology 89, 285-295. Specificity of two widespread Trypanosoma cruzi clonal genotypes or "clonets" (20 and 39) was first analyzed by hybridization with a large set of T. cruzi stocks characterized by multigenic study relying on both MLEE and RAPD. Then, these clonets were detected in the blood of Chagasic children from a Bolivian endemic area by a combination of polymerase chain reaction and clonet-specific DNA hybridization. The distribution of these clonets in patients was significantly different from that observed in the vectors of the same area (Triatoma infestans). In vectors, clonets 20 and 39 are found with comparable frequencies (0.69 and 0.67, respectively) in contrast with patients, in whom clonet 20 and mixed infections exhibit low frequencies. The Chagasic population can be divided into acute infections and latent infections above the accepted criterion of parasitemia (direct microscopic examination). The results suggest a limited selection in the transmission of the two clonets and a further drastic control of clonet 20 parasitemia by the immune system of children patients. (C) 1998 Academic Press.