Specific primers for Xanthomonas vesicatoria, a tomato bacterial spot causal agent

Specific primers for Xanthomonas vesicatoria, a tomato bacterial spot causal agent
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DOI:
10.1007/s10658-013-0225-4
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发表时间:
2013-09-01
影响因子:
1.8
通讯作者:
Quezado-Duval, Alice Maria
Quezado-Duval, Alice Maria
中科院分区:
农林科学3区
文献类型:
--
作者:
Araujo, Edivanio R.;Ferreira, Marisa A. S. V.;Quezado-Duval, Alice Maria

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水泡黄单胞菌(Xanthomonasvesicatoria)是与番茄细菌性斑点病相关的种复合体的成员。新的X. vesicatoria的开发和验证。引物具有高度特异性,使用纯化的细菌DNA、细菌悬液和叶片病变检测呈阳性。这些引物代表了用于检测和鉴定参与这一重要疾病复合体的物种之一的额外工具。
Xanthomonas vesicatoria is a member of the species complex associated with tomato bacterial spot. New and specific primers for X. vesicatoria were developed and validated. The primers were highly specific and detection was positive using purified bacterial DNA, bacterial suspensions and foliar lesions. These primers represent an additional tool for detection and identification of one of the species involved in this important disease complex.