Submicrogram quantitation of an acidic polysaccharide by rocket immunoelectrophoresis and rocket affinoelectrophoresis.
Submicrogram quantitation of an acidic polysaccharide by rocket immunoelectrophoresis and rocket affinoelectrophoresis.
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通过火箭免疫电泳和火箭亲和电泳对酸性多糖进行亚微克定量。
DOI：
10.1016/0003-2697(76)90137-8
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发表时间：
1976
影响因子：
2.9
通讯作者：
M. Salton
中科院分区：
文献类型：
--
作者：
P. Owen;M. Salton
火箭免疫电泳已用于定量从溶壁微球菌膜中分离出的酸性多糖（琥珀酰化脂甘露聚糖）。该程序的灵敏度优于碳水化合物的标准比色测定，并且可以检测低至 10 ng 的甘露聚糖。观察到的火箭高度与抗体和抗原浓度之间的关系遵循 Weeke 对蛋白质抗原所描述的关系 [Weeke, B. (1973)Scand。 J.Immunol.2，增刊。 1, 37–46]。此外，在通过火箭电泳定量这种多糖时，凝集素伴刀豆球蛋白 A 可以用作免疫血清的替代品。然而，这种修饰（火箭亲和电泳）似乎不如火箭免疫电泳灵敏，可检测约 25 ng 甘露聚糖。
Rocket immunoelectrophoresis has been used to quantitate an acidic polysaccharide (a succinylated lipomannan) isolated from the membranes of the bacterium, Micrococcus lysodeikticus. This procedure is superior in sensitivity to standard colorimetric assays for carbohydrate and allows the detection of as little as 10 ng of mannan. The observed relationships between rocket height and both antibody and antigen concentrations follow those described by Weeke for protein antigens [Weeke, B. (1973)Scand. J. Immunol.2, Suppl. 1, 37–46]. Furthermore, the lectin concanavalin A could be used as an alternative to immune serum in the quantitation of this polysaccharide by rocket electrophoresis. However, this modification (rocket affinoelectrophoresis) appeared to be less sensitive than rocket immunoelectrophoresis, allowing the detection of about 25 ng of mannan.