The combined action of hyaluronic acid and fibronectin stimulates neutrophil migration.
The combined action of hyaluronic acid and fibronectin stimulates neutrophil migration.
复制标题
透明质酸和纤连蛋白的联合作用刺激中性粒细胞迁移。
DOI：
10.4049/jimmunol.135.4.2735
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发表时间：
1985
影响因子：
4.4
通讯作者：
P. Venge
中科院分区：
文献类型：
--
作者：
L. Håkansson;P. Venge
以往的研究表明，当血清存在时，透明质酸（HA）可刺激多形核白细胞（PMN）的趋化反应。本研究的目的是确定HA刺激中性粒细胞趋化性所必需的血清因子。通过凝胶过滤法测定了分子量。血清成分的含量超过35万。血清与抗纤连蛋白的免疫沉淀，但不与抗IgM和抗α 2-巨球蛋白，抑制中性粒细胞趋化性的刺激HA。用HA（10 ~ 500 μ g/L）和纤连蛋白（0.1 ~ 100 mg/L）预孵育PMN，可显著刺激PMN的趋化反应。此外，随机迁移的中性粒细胞显着增加与HA（10至500微克/L）和分离的纤连蛋白（50至200毫克/L）的细胞预孵育。此外，用HA（10 - 50 μ g/L）和随后加入的纤连蛋白（10 - 50 mg/L）预孵育的PMN，以及用纤连蛋白（10 mg/L）和在预孵育后加入的HA（5 - 10 μ g/L）预孵育的PMN，显示出对趋化反应的显著刺激。PMN预孵育血清和硫酸软骨素，或与纤维蛋白原和HA，表现出没有刺激的趋化反应。目前的调查表明，HA和纤连蛋白，这可能发生在细胞膜上的联合行动，是在HA介导的刺激中性粒细胞迁移的主要机制。
Previous investigations have demonstrated that the chemotactic response of polymorphonuclear leukocytes (PMN) was stimulated by hyaluronic acid (HA) when serum was present. The aim of the present investigation was to identify the serum factor necessary for the stimulation of PMN chemotaxis by HA. By means of gel filtration, the m.w. of the serum component was shown to be greater than 350,000. Immunoprecipitation of serum with anti-fibronectin, but not with anti-IgM and anti-alpha 2-macroglobulin, inhibited the stimulation of PMN chemotaxis by HA. Preincubation of PMN with HA (10 to 500 micrograms/L) and isolated fibronectin (0.1 to 100 mg/L) significantly stimulated the chemotactic response of PMN. Also, random migration of PMN was significantly increased by preincubation of the cells with HA (10 to 500 micrograms/L) and isolated fibronectin (50 to 200 mg/L). Additionally, PMN preincubated with HA (10 to 50 micrograms/L) and with fibronectin (10 to 50 mg/L) added afterwards, and PMN preincubated with fibronectin (10 mg/L) and with HA (5 to 10 micrograms/L) added after the preincubation, showed a significant stimulation of the chemotactic response. PMN preincubated with serum and chondroitin sulfate, or with fibrinogen and HA, demonstrated no stimulation of the chemotactic response. The present investigation suggests that the combined action of HA and fibronectin, which probably takes place at the cellular membrane, is a major mechanism in the HA-mediated stimulation of PMN migration.