Increase of organic solvent tolerance by overexpression of manXYZ in Escherichia coli
Increase of organic solvent tolerance by overexpression of manXYZ in Escherichia coli
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DOI：
10.1007/s00253-006-0624-y
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发表时间：
2007-01-01
影响因子：
5
通讯作者：
Honda, Hiroyuki
中科院分区：
文献类型：
--
作者：
Okochi, Mina;Kurimoto, Masaki;Honda, Hiroyuki
转录分析已被研究来检测与有机溶剂耐受性相关的基因。大肠杆菌暴露于有机溶剂后的基因表达谱的时间过程显示，manX、manY 和 manZ 基因的表达水平在 2 分钟后分别强烈上调 13.2、10.0 和 7.0 倍，然后在 10 分钟后下降。通过诱导编码磷酸转移酶系统糖转运蛋白的 manX、manY 和 manZ 基因以及 manXYZ 操纵子的过度表达来研究大肠杆菌的有机溶剂耐受性。尽管单独表达manX、manY和manZ并不有效，但通过manXYZ的表达提高了有机溶剂耐受水平。与己烷一起孵育 4 小时后，过表达 manXYZ 的大肠杆菌细胞中的细胞内己烷水平保持较低。
Transcriptional analysis has been investigated to detect the genes involved in organic solvent tolerance. A time course of gene expression profiles of Escherichia coli after exposure to organic solvents revealed that the expression levels of manX, manY, and manZ genes were strongly upregulated by 13.2-, 10.0-, and 7.0-folds, respectively, after 2 min and then decreased after 10 min. Organic solvent tolerance of E. coli was investigated by inducing overexpression of manX, manY, and manZ genes and manXYZ operon that encode a sugar transporter of the phosphotransferase system. Although the expression of manX, manY, and manZ alone was not effective, the organic solvent tolerance level was increased by the expression of manXYZ. The intracellular hexane level was kept lower in E. coli cells overexpressing manXYZ after 4 h of incubation with hexane.