Correlation of phospholipid hydroperoxide glutathione peroxidase activity to the sensitivity of rat retinas to photic injury.
Correlation of phospholipid hydroperoxide glutathione peroxidase activity to the sensitivity of rat retinas to photic injury.
复制标题
磷脂氢过氧化物谷胱甘肽过氧化物酶活性与大鼠视网膜对光损伤敏感性的相关性。
DOI：
10.1159/000267376
复制
发表时间：
1994
影响因子：
2.1
通讯作者：
Tso,MO
中科院分区：
文献类型：
--
作者：
Wang,L;Lam,TT;Lam,KW;Tso,MO
我们推测，不同品系的大鼠视网膜之间的光损伤的差异敏感性可能取决于水平的磷脂氢过氧化物谷胱甘肽过氧化物酶（PHGPX）的活性，在视网膜中的内源性抗氧化酶之一。以过氧化氢、氢过氧化枯烯和过氧化磷脂为检测底物，分析了4种近交系白化病大鼠（Fischer、Wistar、布法罗和刘易斯）视网膜的谷胱甘肽过氧化物酶活性。在所有四种品系的大鼠中，PHGPX仅在视网膜的高盐提取物中观察到，而H2 O2或氢过氧化枯烯测定的过氧化物酶主要在低盐提取物中观察到。PHGPX在最耐光的Fischer品系中最高（66.7 mU/mg），在最光敏感的刘易斯品系中最低（31.9 mU/mg），而在中等光敏感的布法罗和Wistar品系中的活性水平分别为46.6和38.5 mU/mg。而H2 O2法和过氧化氢异丙苯法测定的过氧化物酶活性在4株菌间差异不显著。这些观察结果表明，在大鼠视网膜，膜相关的PHGPX可能有一个重要的作用，对光诱导的自由基损伤的防御。
We hypothesize that the differential susceptibility to photic injury among different strains of rat retinas may depend on the levels of phospholipid hydroperoxide glutathione peroxidase (PHGPX) activity, one of the endogenous antioxidant enzymes in the retina. The retinas of four inbred strains of albino rat (Fischer, Wistar, Buffalo and Lewis) were analyzed for glutathione peroxidase activity using H2O2, cumene hydroperoxide, and phospholipid hydroperoxide as assay substrates. In all four strains of rat, PHGPX was observed only in the high salt extract of the retina, while peroxidases determined by H2O2or cumene hydroperoxide were observed mainly in the low salt extract. PHGPX was highest (66.7 mU/mg) in the most light-resistant Fischer strain and lowest in the most light-sensitive Lewis strain (31.9 mU/mg), while the activity levels in the moderately light-sensitive Buffalo and Wistar strains were 46.6 and 38.5 mU/mg, respectively. In contrast, there was no significant difference in peroxidases determined by H2O2or cumene hydroperoxide among the four strains. These observations suggested that, in rat retina, the membrane-associated PHGPX may have an important role in the defense against light-induced free radical damage.