Multidimensional analysis of actin depolymerising factor family in pigeon pea under different environmental stress revealed specific response genes in each subgroup
Multidimensional analysis of actin depolymerising factor family in pigeon pea under different environmental stress revealed specific response genes in each subgroup
复制标题
不同环境胁迫下木豆肌动蛋白解聚因子家族的多维分析揭示了每个亚群的特定响应基因
DOI：
10.1071/fp20190
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发表时间：
2020-09-25
影响因子：
3
通讯作者：
Fu, Yujie
中科院分区：
文献类型：
--
作者：
Cao, Hongyan;Amin, Rohul;Fu, Yujie
肌动蛋白解聚因子（ADF）是一种普遍存在于动物和植物细胞中的肌动蛋白结合蛋白。它在植物生长发育以及抵抗生物和非生物胁迫方面发挥着重要作用。植物ADF家族的研究仅限于拟南芥(Arabidopsis thaliana(L.) Heynh)。和一些草本作物，但迄今为止尚未有木本经济作物的报道。从木豆基因组中鉴定出Cajanus cajan(L.) Millsp.ADF(CcADF)家族的所有成员，并通过系统发育分析将其分布在四个亚科中。CcADF在基因结构进化、蛋白质结构和功能表达方面相对保守，不同的CcADF在不同处理下表现出特定的表达模式。通过分析CcADF的应激响应模式和铝胁迫的时间序列RNA-seq，揭示了几个关键CcADF的表达特征。其中，第一亚组中的CcADF9对根部的铝胁迫有特异性反应；第二亚组中的CcADF3对叶片中的真菌感染有强烈的反应；第四亚组中的CcADF2对不同组织中的各种应激治疗有积极反应。这项研究扩展了植物ADF家族与铝耐受性之间的关系，并增加了对木本作物CcADF家族的了解。
Actin depolymerising factor (ADF) is an actin binding protein that is ubiquitous in animal and plant cells. It plays an important role in plant growth and development, as well as resistance to biotic and abiotic stress. The research of plant ADF family has been restricted toArabidopsis thaliana(L.) Heynh. and some herb crops, but no woody cash crops have been reported to date. All members of theCajanus cajan(L.) Millsp.ADF(CcADF) family were identified from the pigeon pea genome, and distributed among the four subfamilies by phylogenetic analysis.CcADFswere relatively conservative in gene structure evolution, protein structure and functional expression, and differentCcADFsshowed specific expression patterns under different treatments. The expression characteristics of several keyCcADFswere revealed by analysing the stress response pattern ofCcADFsand the time series RNA-seq of aluminium stress. Among them,CcADF9in the first subgroup specifically responded to aluminium stress in the roots;CcADF3in the second subgroup intensively responded to fungal infection in the leaves; andCcADF2in the fourth subgroup positively responded to various stress treatments in different tissues. This study extended the relationship between plantADFfamily and aluminium tolerance, as well as adding to the understanding ofCcADFfamily in woody crops.