ENTRANCE OF GLYCEROL INTO PLASMALOGENS OF SOME STRICTLY ANAEROBIC BACTERIA AND PROTOZOA
ENTRANCE OF GLYCEROL INTO PLASMALOGENS OF SOME STRICTLY ANAEROBIC BACTERIA AND PROTOZOA
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DOI：
10.1016/0014-5793(76)80204-9
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发表时间：
1976-01-01
期刊：
影响因子：
3.5
通讯作者：
VANGOLDE, LMG
中科院分区：
文献类型：
--
作者：
PRINS, RA;VANGOLDE, LMG
哺乳动物组织中醚类脂质生物合成的途径已被大量阐明[1 -3]。对于我们目前对这些途径的理解来说，最重要的是Wykle和Snyder[5]以及Hajra[5]的观察，即磷酸二羟丙酮，或者更确切地说是酰基磷酸二羟丙酮，是烷基和烷基-1-烯基脂质的甘油部分的特异性前体。1-Alk-1 ' -乙烯-2-酰基磷酸甘油酯（plasmalogen）在许多严格厌氧细菌[6-81]和原生动物[bbb]中大量存在。然而，我们对这些严格厌氧微生物中缩醛原形成机制的了解仍然非常少。Hill和Lands [lo]将[2-3H]和[3- ' 4C]甘油加入到丁酸梭菌的总1,2 -二酰基1-烷基- 1 ' -酰基-2-酰基磷酸甘油酯组分中。将甘油掺入缩醛原后，甘油2位的3H不稳定。实际上，发现缩醛原的3H/14C比提供给培养基的甘油高。根据这一观察，Hill和Lands得出结论，在C: butyricum中，甘油不像在哺乳动物组织中观察到的那样，通过磷酸二羟丙酮进入磷脂原[4,5]。然而，Hill and Lands的做法受到了Manning和Brindley的批评[11]。这些作者提供的证据表明，至少对于肝脏，通过磷酸二羟丙酮掺入甘油的相对重要性的真实估计只能是
The pathways involved in the biosynthesis of ether lipids in mammalian tissues have been largely elucidated [l-3]. Of paramount importance for our current understanding of these pathways was the observation by Wykle and Snyder [4] and by Hajra [5] that dihydroxyacetone phosphate, or rather acyldihydroxyacetone phosphate, is the obligate precursor of the glycerol portion of alkyl-and alk-1-enyl lipids. 1-Alk-1 ‘-enyl-2-acyl phosphoglycerides (plasmalogens) occur in abundant amounts in many strictly anaerobic bacteria [6-81 and protozoa [9]. Our knowledge of the mechanisms operating in the formation of plasmalogens in these strictly anaerobic micro-organisms is, however, still very scarce [6].Hill and Lands [lo] followed the incorporation of [2-3H]-and [3-‘4C] glycero1 into the total 1, 2-diacyland 1-alk-l’-enyl-2-acyl phosphoglyceride fractions of Clostridium butyricum. The 3H at position 2 of the glycerol was not labilized upon incorporation of glycerol into plasmalogens. Actually, the 3H/14C ratio of the plasmalogens was found to be higher than that of the glycerol supplied to the medium. From this observation Hill and Lands concluded that in C: butyricum glycerol does not enter into plasmalogens via dihydroxyacetone phosphate as had been observed for mammalian tissues [4, 5]. However, the approach of Hill and Lands [lo] was criticized by Manning and Brindley [1 l]. These authors provided evidence that, at least for the liver, a true estimate of the relative importance of the incorporation of glycerol via dihydroxyacetone phosphate can only be