Long-term culture of human lung adenocarcinoma A549 cells enhances the replication of human influenza A viruses.
Long-term culture of human lung adenocarcinoma A549 cells enhances the replication of human influenza A viruses.
复制标题
人肺腺癌A549细胞的长期培养增强了人甲型流感病毒的复制。
DOI：
10.1099/jgv.0.001314
复制
发表时间：
2019
期刊：
影响因子：
3.8
通讯作者：
Kawaoka Y.
中科院分区：
文献类型：
--
作者：
Ujie M;Takada K;Kiso M;Sakai-Tagawa Y;Ito M;Nakamura K;Watanabe S;Imai M;Kawaoka Y.
人肺腺癌细胞系 A549 的长期培养可促进这些细胞向肺泡 II 型细胞表型分化。在这里，我们评估了长期培养的 A549 细胞对人类流感病毒的敏感性。 A549细胞在Ham’s F12K培养基中连续培养25天（D25-A549）或1天（D1-A549）。六种人类甲型流感病毒在 D25-A549 细胞中的生长速度比在 D1-A549 细胞中快得多；然而，两种乙型流感病毒在两种细胞类型中复制都很差。两种禽流感病毒在两种细胞类型中都能有效复制，滴度相似。 D25-A549 细胞中人病毒受体的表达水平高于 D1-A549 细胞。因此，与 D1-A549 细胞相比，D25-A549 细胞更有效地支持人甲型流感病毒的复制。我们的数据表明，长期培养的 A549 细胞将有助于甲型流感病毒研究。
Long-term culture of the human lung adenocarcinoma cell line A549 promotes the differentiation of these cells toward an alveolar type II cell phenotype. Here, we evaluated the susceptibility of long-term cultured A549 cells to human influenza viruses. A549 cells were cultured continuously for 25 days (D25-A549) or 1 day (D1-A549) in Ham’s F12K medium. Six human influenza A viruses grew much faster in D25-A549 cells than in D1-A549 cells; however, two influenza B viruses replicated poorly in both cell types. Two avian influenza viruses replicated efficiently in both cell types, with similar titres. Expression levels of human virus receptors were higher in D25-A549 cells than in D1-A549 cells. D25-A549 cells thus more efficiently support the replication of human influenza A viruses compared with D1-A549 cells. Our data suggest that long-term cultured A549 cells will be useful for influenza A virus research.