Alterations of regional cerebral blood flow and oxygen saturation in a rat sinus-vein thrombosis model
Alterations of regional cerebral blood flow and oxygen saturation in a rat sinus-vein thrombosis model
复制标题
DOI：
10.1161/01.str.27.4.720
复制
发表时间：
1996-04-01
期刊：
影响因子：
8.3
通讯作者：
Kempski, O
中科院分区：
文献类型：
--
作者：
Nakase, H;Heimann, A;Kempski, O
背景和目的：对患者和实验动物的窦静脉血栓形成（SVT）的病理生理机制仍知之甚少。本研究旨在检查和进一步阐明事件的病理生理序列，特别是在相同位置检测到的局部和区域血液流动和血红蛋白氧饱和度（HbSO（2））之间的关系。方法结扎大鼠上级矢状窦（SSS），向SSS内缓慢注射高岭土脑磷脂混悬液，建立SVT模型。局部脑血流量（rCBF）通过激光多普勒血流仪和局部HbSO（2）进行评估，局部HbSO（2）在SVT后90分钟内通过显微分光光度计技术在48个相同位置进行测量。在SVT诱导前和诱导后30和90分钟进行荧光血管造影。结果荧光血管造影结果可将动物分为3组：（1）孤立性上矢状窦血栓组（A组，n=8）;（2）上矢状窦和皮质静脉血栓组（B组，n=10）;（3）假手术组（C组，n=5）。B组rCBF、HbSO（2）下降，脑组织损伤明显，A组无明显变化。局部HbSO（2）的降低先于矢状窦结扎后的血流减少，但在血栓形成之前。A组SVT后血压明显高于B组和C组。结论上矢状窦血栓急性扩展至皮质静脉的脑内血液和氧气供应严重减少。CBF、局部HbSO（2）和重复血管造影可作为SVT后早期发现危重情况的有用监测。局部HbSO（2）比1CBF对预测结果更敏感。此外，旨在改善灌注压或降低血管阻力的治疗可在SVT进展期间打开进一步的治疗窗口。
Background and Purpose The pathophysiology of sinus-vein thrombosis (SVT) in patients and experimental animals is still poorly understood. This study was designed to examine and further elucidate the pathophysiological sequence of events, especially the relationship between local and regional blood how and hemoglobin oxygen saturation (HbSO(2)) detected at identical locations. The use of both parameters as outcome indicators should be compared.Methods SVT was induced by ligation of the superior sagittal sinus (SSS) and slow injection of kaolin-cephalin suspension into the SSS in rats. Regional cerebral blood flow (rCBF) was assessed by laser-Doppler flowmetry together with regional HbSO(2), which was measured by a microspectrophotometric technique at 48 identical locations for 90 minutes after SVT using a scanning technique. Fluorescence angiography was performed before and 30 and 90 minutes after SVT induction. After 48 hours the animals were killed for histology.Results The fluorescence angiographic findings could divide animals into three groups: (1) group A, with a solitary SSS thrombus (n=8); (2) group B, with a thrombosis of SSS and cortical veins (n=10); (3) group C, animals that had undergone sham operation (n=5). Decreases of rCBF and HbSO(2) and brain damage were seen in group B but not in group A. The reduction of local HbSO(2) preceded the flow decrease after sagittal sinus ligation but before thrombosis. Blood pressure in group A was found to be significantly higher after SVT than in groups B and C.Conclusions The brain with acute extension of a thrombus from the SSS into cortical veins experiences a critically reduced supply of blood and oxygen. CBF, local HbSO(2), and repeated angiography can be helpful monitors for the early detection of critical conditions after SVT. Local HbSO(2) has a greater sensitivity to predict outcome than 1CBF. Moreover, therapies directed to improve perfusion pressure or reduce vascular resistance may open further therapeutic windows during SVT progression.